Protocols shared by authors via RaP service
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The luciferase reporter Sec UGA read through assay
Authors: Benjamin Yee Killam, Miljan Simonović and Paul R Copeland
Date: Aug 8, 2022，Views：560
Original protocol used in research article：
Structure of the mammalian ribosome as it decodes the selenocysteine UGA codon
Science, Jun 16, 2022; DOI: 10.1126/science.abg3875
Neonatal odor conditioning
Author: Hitoshi Sakano
Date: Aug 6, 2022，Views：555
Original protocol used in research article：
The olfactory critical period is determined by activity-dependent Sema7A/PlxnC1 signaling within glomeruli
eLife, Mar 29, 2021; DOI: 10.7554/eLife.65078
PKG-dependent TSC2 phosphorylation
Author: David A Kass
Date: Aug 5, 2022，Views：558
Original protocol used in research article：
Single serine on TSC2 exerts biased control over mTORC1 activation mediated by ERK1/2 but not Akt
Life Science Alliance, Mar 14, 2022; DOI: 10.26508/lsa.202101169
Primary source gender and cell line confirmations
Authors: Eben G Estell and Clifford J Rosen
Date: Aug 5, 2022，Views：575
Original protocol used in research article：
Irisin directly stimulates osteoclastogenesis and bone resorption in vitro and in vivo
eLife, Aug 11, 2020; DOI: 10.7554/eLife.58172
ISH and IHC
Authors: Minh Q Nguyen, Nicholas JP Ryba and Steve Davidson
Date: Aug 5, 2022，Views：606
Original protocol used in research article：
Single-nucleus transcriptomic analysis of human dorsal root ganglion neurons
eLife, Nov 26, 2021; DOI: 10.7554/eLife.71752
Test on generated test data
Authors: Sebastian Fürthauer and Stefanie Redemann
Date: Aug 4, 2022，Views：590
Original protocol used in research article：
Microtubule reorganization during female meiosis in C. elegans
eLife, Jun 11, 2021; DOI: 10.7554/eLife.58903
Exercise training
Authors: Alyson Sujkowski, Robert J Wessells and Sokol V Todi
Date: Aug 4, 2022，Views：701
Original protocol used in research article：
Endurance exercise ameliorates phenotypes in Drosophila models of spinocerebellar ataxias
eLife, Feb 16, 2022; DOI: 10.7554/eLife.75389
Calculating David's Score
Authors: Katherine B LeClair and Scott J Russo
Date: Aug 2, 2022，Views：1016
Original protocol used in research article：
Individual history of winning and hierarchy landscape influence stress susceptibility in mice
eLife, Sep 28, 2021; DOI: 10.7554/eLife.71401
HiCRep analysis
Authors: Sarah G Swygert and Toshio Tsukiyama
Date: Aug 2, 2022，Views：560
Original protocol used in research article：
Local chromatin fiber folding represses transcription and loop extrusion in quiescent cells
eLife, Nov 4, 2021; DOI: 10.7554/eLife.72062
Electrophoretic mobility shift assays (EMSAs)
Authors: Roni M Lahr and Andrea J Berman
Date: Aug 2, 2022，Views：616
Original protocol used in research article：
La-related protein 1 (LARP1) binds the mRNA cap, blocking eIF4F assembly on TOP mRNAs
eLife, Apr 7, 2017; DOI: 10.7554/eLife.24146
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