Protocols shared by authors via RaP service
Journal
TraDIS method.
Authors: Karthik Pullela, Ian R. Henderson, Alessandra Polissi and Waldemar Vollmer
Date: Aug 17, 2022，Views：533
Original protocol used in research article：
DpaA Detaches Braun’s Lipoprotein from Peptidoglycan
mBio, May 4, 2021; DOI: 10.1128/mbio.00836-21
In vitro culture on tissue culture plastic, fibroblasts, or keratinocytes
Authors: Tiago R. Matos, Jessica E. Teague and Rachael A. Clark
Date: Aug 16, 2022，Views：594
Original protocol used in research article：
Central memory T cells are the most effective precursors of resident memory T cells in human skin
Science Immunology, Apr 22, 2022; DOI: 10.1126/sciimmunol.abn1889
Diversity based on T cell composition
Authors: Liangtao Zheng, Shishang Qin, Xueda Hu and Zemin Zhang
Date: Aug 15, 2022，Views：594
Original protocol used in research article：
Pan-cancer single-cell landscape of tumor-infiltrating T cells
Science, Dec 17, 2021; DOI: 10.1126/science.abe6474
Indisulam efficacy studies in in vivo models
Authors: Shivendra Singh, Waise Quarni, Deepak Nijhawan, Andrew M. Davidoff and Jun Yang
Date: Aug 15, 2022，Views：552
Original protocol used in research article：
Targeting the spliceosome through RBM39 degradation results in exceptional responses in high-risk neuroblastoma models
Science Advances, Nov 17, 2021; DOI: 10.1126/sciadv.abj5405
In vitro phase separation assay
Authors: Yu Zhang, Lisha Shen and Hao Yu
Date: Aug 14, 2022，Views：697
Original protocol used in research article：
Phase separation of HRLP regulates flowering time in Arabidopsis
Science Advances, Jun 22, 2022; DOI: 10.1126/sciadv.abn5488
Plaque assays and immunostaining.
Authors: Chengjin Ye and Luis Martinez-Sobrido
Date: Aug 12, 2022，Views：558
Original protocol used in research article：
Generation and Characterization of Recombinant SARS-CoV-2 Expressing Reporter Genes
Journal of Virology, Mar 10, 2021; DOI: 10.1128/JVI.02209-20
RNA sequencing, RNA immunoprecipitation, and plasmid barcode sequencing
Authors: James Hose and Audrey P Gasch
Date: Aug 12, 2022，Views：566
Original protocol used in research article：
The genetic basis of aneuploidy tolerance in wild yeast
eLife, Jan 7, 2020; DOI: 10.7554/eLife.52063
Statistical analysis
Authors: Elisa Scarselli and Luigia Pace
Date: Aug 12, 2022，Views：533
Original protocol used in research article：
Adenoviral-based vaccine promotes neoantigen-specific CD8+ T cell stemness and tumor rejection
Science Translational Medicine, Aug 10, 2022; DOI: 10.1126/scitranslmed.abo7604
Flow Cytometry
Authors: Demi Brownlie and Nicole Marquardt
Date: Aug 11, 2022，Views：543
Original protocol used in research article：
Comparison of Lung-Homing Receptor Expression and Activation Profiles on NK Cell and T Cell Subsets in COVID-19 and Influenza
Frontiers in Immunology, Mar 16, 2022; DOI: 10.3389/fimmu.2022.834862
Microscale thermophoresis
Authors: Yanglin Ding and Shuhua Yang
Date: Aug 10, 2022，Views：555
Original protocol used in research article：
CPK28-NLP7 module integrates cold-induced Ca2+ signal and transcriptional reprogramming in Arabidopsis
Science Advances, Jun 29, 2022; DOI: 10.1126/sciadv.abn7901
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