Stem Cell

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    Absolute Quantification of mRNA Isoforms in Adult Stem Cells Using Microfluidic Digital PCR
    Authors:  Shubhangi Das Barman, Zofija Frimand and Antoine de Morree, date: 09/05/2023, view: 165, Q&A: 0

    Adult stem cells play key roles in homeostasis and tissue repair. These cells are regulated by a tight control of transcriptional programs. For example, muscle stem cells (MuSCs), located beneath the basal lamina, exist in the quiescent state but

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    Preparation of Human Kidney Progenitor Cultures and Their Differentiation into Podocytes

    Kidney diseases are a global health concern. Modeling of kidney disease for translational research is often challenging because of species specificities or the postmitotic status of kidney epithelial cells that make primary cultures, for example

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    A New Approach to Generate Gastruloids to Develop Anterior Neural Tissues
    Authors:  Mehmet Girgin, Nicolas Broguiere, Lorenzo Mattolini and Matthias Lutolf, date: 07/20/2023, view: 678, Q&A: 0

    Embryonic development is a complex process integrating cell fate decisions and morphogenesis in a spatiotemporally controlled manner. Previous studies with model organisms laid the foundation of our knowledge on post-implantation development;

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    Automated 384-well SYBR Green Expression Array for Optimization of Human Induced Pluripotent Stem Cell Differentiation
    Authors:  Max Y. Chen, Laurin Heinrich, Faria Zafar, Kamilla Sedov and Birgitt Schuele, date: 06/05/2023, view: 545, Q&A: 0

    Cell populations and tissues exhibit unique gene expression profiles, which allow for characterizing and distinguishing cellular subtypes. Monitoring gene expression of cell type–specific markers can indicate cell status such as proliferation,

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    Establishing Bipotential Human Lung Organoid Culture System and Differentiation to Generate Mature Alveolar and Airway Organoids
    Authors:  Man Chun Chiu, Cun Li, Yifei Yu, Xiaojuan Liu, Jingjing Huang, Zhixin Wan, Kwok Yung Yuen and Jie Zhou, date: 04/20/2023, view: 763, Q&A: 0

    A robust in vitro model of the human respiratory epithelium, including the alveolar and the airway epithelium, is essential for understanding the biology and pathology of the human respiratory system. We previously described a protocol to derive

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    Determining Bone-forming Ability and Frequency of Skeletal Stem Cells by Kidney Capsule Transplantation and Limiting Dilution Assay
    Authors:  Hitoshi Uchida, Takamitsu Maruyama and Wei Hsu, date: 03/20/2023, view: 201, Q&A: 0

    Adult stem cells not only maintain tissue homeostasis but are also critical for tissue regeneration during injury. Skeletal stem cells are multipotent stem cells that can even generate bones and cartilage upon transplantation to an ectopic site. ...

    Laser Capture Microdissection (LCM) of Human Skin Sample for Spatial Proteomics Research
    Authors:  Qiyu Zhang, Huizi Gong, Jie Ma, Jun Li and Ling Leng, date: 03/05/2023, view: 494, Q&A: 0

    In mammals, the skin comprises several distinct cell populations that are organized into the following layers: epidermis (stratum corneum, stratum granulosum, stratum spinosum, and basal layer), basement membrane, dermis, and hypodermal

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    Thrombopoietin-independent Megakaryocyte Differentiation of Hematopoietic Progenitor Cells from Patients with Myeloproliferative Neoplasms

    Primary hematopoietic stem and progenitor cell (HSPC)-derived megakaryocytes are a valuable tool for translational research interrogating disease pathogenesis and developing new therapeutic avenues for patients with hematologic disorders including

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    Using BODIPY FL-Sphingolipid Analogs to Study Sphingolipid Metabolism in Mouse Embryonic Stem Cells
    Authors:  Wei Fan and Xiaoling Li, date: 11/20/2022, view: 671, Q&A: 0

    Sphingolipids are important structural components of cellular membranes. They also function as prominent signaling molecules to control a variety of cellular events, such as cell growth, differentiation, and apoptosis. Impaired sphingolipid

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    Generation of iMyoblasts from Human Induced Pluripotent Stem Cells

    Skeletal muscle stem cells differentiated from human-induced pluripotent stem cells (hiPSCs) serve as a uniquely promising model system for investigating human myogenesis and disease pathogenesis, and for the development of gene editing and

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