H2 Production from Methyl Viologen–Dependent Hydrogenase Activity Monitored by Gas Chromatography
    Author:  Nuttavut Kosem, date: 12/05/2023, view: 61, Q&A: 0

    Bio-hydrogen production is an eco-friendly alternative to commercial H2 production, taking advantage of natural systems. Microbial hydrogenases play a main role in biological mechanisms, catalyzing proton reduction to molecular hydrogen (H2)

    Purification of Human Cytoplasmic Actins From Saccharomyces cerevisiae
    Authors:  Brian K. Haarer, David C. Amberg and Jessica L. Henty-Ridilla, date: 12/05/2023, view: 34, Q&A: 0

    Eukaryotic cells rely on actin to support cellular structure, motility, transport, and a wide variety of other cytoplasmic functions and nuclear activities. Humans and other mammals express six closely related isoforms of actin, four of which are

    Detailed Protocol to Perform Direct PCR Using Filamentous Fungal Biomass—Tips and Considerations
    Authors:  Hosung Jeon, Hokyoung Son and Kyunghun Min, date: 11/05/2023, view: 197, Q&A: 0

    The precise and rapid detection of fungi is important in various fields, including clinics, industry, and agriculture. While sequencing universal DNA barcodes remains the standard method for species identification and phylogenetic analysis, a

    Spot Assay and Colony Forming Unit (CFU) Analyses–based sensitivity test for Candida albicans and Saccharomyces cerevisiae

    Cellular sensitivity is an approach to inhibit the growth of certain cells in response to any non-permissible conditions, as the presence of a cytotoxic agent or due to changes in growth parameters such as temperature, salt, or media components.

    A Guideline for Assessment and Characterization of Bacterial Biofilm Formation in the Presence of Inhibitory Compounds
    Authors:  Bassam A. Elgamoudi and Victoria Korolik, date: 11/05/2023, view: 188, Q&A: 0

    Campylobacter jejuni, a zoonotic foodborne pathogen, is the worldwide leading cause of acute human bacterial gastroenteritis. Biofilms are a significant reservoir for survival and transmission of this pathogen, contributing to its overall

    Analysis of Plasmodium falciparum Mitochondrial Electron Transport Chain Activity Using Seahorse XFe96 Extracellular Flux Assays

    The mitochondrial electron transport chain (ETC) is a multi-component pathway that mediates the transfer of electrons from metabolic reactions that occur in the mitochondrion to molecular oxygen (O2). The ETC contributes to numerous cellular

    Workflow for High-throughput Screening of Enzyme Mutant Libraries Using Matrix-assisted Laser Desorption/Ionization Mass Spectrometry Analysis of Escherichia coli Colonies
    Authors:  Kisurb Choe and Jonathan V. Sweedler, date: 11/05/2023, view: 279, Q&A: 0

    High-throughput molecular screening of microbial colonies and DNA libraries are critical procedures that enable applications such as directed evolution, functional genomics, microbial identification, and creation of engineered microbial strains to

    Cell Cycle Analysis of Candida albicans by Flow Cytometry
    Authors:  Shraddheya Kumar Patel, Satya Ranjan Sahu and Narottam Acahrya, date: 10/20/2023, view: 324, Q&A: 0

    The cell cycle is a vital process of cell division that is required to sustain life. Since faithful cell division is critical for the proper growth and development of an organism, the study of the cell cycle becomes a fundamental research objective.

    Production, Extraction, and Solubilization of Exopolysaccharides Using Submerged Cultures of Agaricomycetes

    Macrofungi, also known as mushrooms, can produce various bioactive compounds, including exopolysaccharides (EPS) with distinct biological properties and subsequent industrial applications in the preparation of cosmetics, pharmaceuticals, and food

    Co-culture Wood Block Decay Test with Bacteria and Wood Rotting Fungi to Analyse Synergism/Antagonism during Wood Degradation
    Authors:  Julia Embacher, Susanne Zeilinger, Sigrid Neuhauser and Martin Kirchmair, date: 10/05/2023, view: 202, Q&A: 0

    Mixed communities of fungi and bacteria have been shown to be more efficient in degrading wood than fungi alone. Some standardised protocols for quantification of the wood decay ability of fungi have been developed (e.g., DIN V ENV 12038:2002 as the


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