Protocols shared by authors via RaP service
Journal
Immunofluorescence
Authors: Jeffrey Lee, Peter Wing, Alfredo Castello, Jane A McKeating and Ilan Davis
Date: Sep 5, 2022，Views：593
Original protocol used in research article：
Absolute quantitation of individual SARS-CoV-2 RNA molecules provides a new paradigm for infection dynamics and variant differences
eLife, Jan 20, 2022; DOI: 10.7554/eLife.74153
Isolation by FACS sorting for qRT-PCR of Drosophila larvae class IV da neurons
Authors: Jacob Jaszczak, Laura Devault, Lily Jan and Yuh Nung Jan
Date: Sep 2, 2022，Views：583
Original protocol used in research article：
Steroid hormone signaling activates thermal nociception during Drosophila peripheral nervous system development
eLife, Mar 30, 2022; DOI: 10.7554/eLife.76464
Protein interaction analysis in Y2H system
Authors: Vladimir Denic and Houqing Yu
Date: Sep 1, 2022，Views：746
Original protocol used in research article：
The peroxisomal exportomer directly inhibits phosphoactivation of the pexophagy receptor Atg36 to suppress pexophagy in yeast
eLife, Apr 11, 2022; DOI: 10.7554/eLife.74531
In vitro kinase assay
Authors: Vladimir Denic and Houqing Yu
Date: Sep 1, 2022，Views：1303
Original protocol used in research article：
The peroxisomal exportomer directly inhibits phosphoactivation of the pexophagy receptor Atg36 to suppress pexophagy in yeast
eLife, Apr 11, 2022; DOI: 10.7554/eLife.74531
Adapter ligation and RT-PCR
Authors: Emil Laust Kristoffersen, Agnes Noy and Philipp Holliger
Date: Sep 1, 2022，Views：736
Original protocol used in research article：
Rolling circle RNA synthesis catalyzed by RNA
eLife, Feb 2, 2022; DOI: 10.7554/eLife.75186
mib1_ta52b 4 primer genotyping protocol
Author: Maximilian Fürthauer
Date: Sep 1, 2022，Views：560
Original protocol used in research article：
The E3 ubiquitin ligase mindbomb1 controls planar cell polarity-dependent convergent extension movements during zebrafish gastrulation
eLife, Feb 10, 2022; DOI: 10.7554/eLife.71928
FAK purification
Authors: Lindsay B Case and Michael K Rosen
Date: Aug 31, 2022，Views：576
Original protocol used in research article：
Synergistic phase separation of two pathways promotes integrin clustering and nascent adhesion formation
eLife, Jan 20, 2022; DOI: 10.7554/eLife.72588
Echo-MRI analysis
Authors: Rozalyn M Anderson and Priya Balasubramanian
Date: Aug 31, 2022，Views：634
Original protocol used in research article：
Adiponectin receptor agonist AdipoRon improves skeletal muscle function in aged mice
eLife, Mar 17, 2022; DOI: 10.7554/eLife.71282
APC maturation evaluation
Authors: Mariusz Skwarczynski and Istvan Toth
Date: Aug 30, 2022，Views：564
Original protocol used in research article：
Poly(amino acids) as a potent self-adjuvanting delivery system for peptide-based nanovaccines
Science Advances, Jan 28, 2020; DOI: 10.1126/sciadv.aax2285
Immunoblot analysis
Authors: Sandra Olenic and Lee Kroos
Date: Aug 30, 2022，Views：578
Original protocol used in research article：
Inhibitory proteins block substrate access by occupying the active site cleft of Bacillus subtilis intramembrane protease SpoIVFB
eLife, ; DOI: 10.7554/eLife.74275
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