Cancer Biology


    Reprogramming Cancer Cells to Antigen-presenting Cells

    Cancer cells evade the immune system by downregulating antigen presentation. Although immune checkpoint inhibitors (ICI) and adoptive T-cell therapies revolutionized cancer treatment, their efficacy relies on the intrinsic immunogenicity of tumor

    Studying Cell Migration (Random and Wound Healing) Parameters with Imaging and MATLAB Analysis
    Authors:  Ling-Yea Yu, Hsuan-Chao Lin, Chi-Lin Hsu, Tuan-Yu Kao and Feng-Chiao Tsai, date: 11/05/2023, view: 129, Q&A: 0

    Cell migration is an essential biological process for organisms, in processes including embryonic development, immune response, and cancer metastasis. To elucidate the regulatory machinery of this vital process, methods that mimic in vivo migration,

    Studying Cellular Focal Adhesion Parameters with Imaging and MATLAB Analysis

    Cell signaling is highly integrated for the process of various cell activities. Although previous studies have shown how individual genes contribute to cell migration, it remains unclear how the integration of these signaling pathways is involved in

    Microscopy and Plate Reader–based Methods for Monitoring the Interaction of Platelets and Tumor Cells in vitro
    Authors:  Veeresh Toragall, Elizabeth J. Hale, Kenneth R. Hulugalla and Thomas A. Werfel, date: 10/20/2023, view: 611, Q&A: 0

    Platelets and their activation status play an essential role in cancer metastasis. Therefore, the anti-metastatic potential of antiplatelet drugs has been investigated for many years. However, the initial screening of these antiplatelet drugs to

    Efficient Large DNA Fragment Knock-in by Long dsDNA with 3′-Overhangs Mediated CRISPR Knock-in (LOCK) in Mammalian Cells
    Authors:  Wenjie Han, Haojun Liang and Jianqiang Bao, date: 10/20/2023, view: 620, Q&A: 0

    An efficient and precise genome-editing approach is in high demand in any molecular biology or cell biology laboratory worldwide. However, despite a recent rapid progress in the toolbox tailored for precise genome-editing, including the base editors

    Quantification of Chromosomal Aberrations in Mammalian Cells
    Authors:  Inés Paniagua and Jacqueline J. L. Jacobs, date: 08/20/2023, view: 728, Q&A: 0

    Maintenance of genome integrity requires efficient and faithful resolution of DNA breaks and DNA replication obstacles. Dysfunctions in any of the processes orchestrating such resolution can lead to chromosomal instability, which appears as

    Ex vivo Drug Sensitivity Imaging-based Platform for Primary Acute Lymphoblastic Leukemia Cells

    Resistance of acute lymphoblastic leukemia (ALL) cells to chemotherapy, whether present at diagnosis or acquired during treatment, is a major cause of treatment failure. Primary ALL cells are accessible for drug sensitivity testing at the time of

    Isoform-specific, Semi-quantitative Determination of Highly Homologous Protein Levels via CRISPR-Cas9-mediated HiBiT Tagging
    Authors:  Kristina Seiler, Sreoshee Rafiq and Mario P. Tschan, date: 07/20/2023, view: 634, Q&A: 0

    Many protein families consist of multiple highly homologous proteins, whether they are encoded by different genes or originating from the same genomic location. Predominance of certain isoforms has been linked to various pathological conditions,

    mRNA Delivery Platform Based on Bacterial Outer Membrane Vesicles for Tumor Vaccine
    Authors:  Xiaoyu Gao, Yao Li, Guangjun Nie and Xiao Zhao, date: 07/05/2023, view: 566, Q&A: 0

    The rapid display and delivery method for customized tumor mRNA vaccines is limited. Herein, bacteria-derived outer membrane vesicles (OMVs) are employed as an mRNA delivery platform by surface engineering of an RNA-binding protein, L7Ae. OMV-L7Ae

    3D Ultrastructural Visualization of Mitosis Fidelity in Human Cells Using Serial Block Face Scanning Electron Microscopy (SBF-SEM)
    Authors:  Nuria Ferrandiz and Stephen J. Royle, date: 07/05/2023, view: 484, Q&A: 0

    Errors in chromosome segregation during mitosis lead to chromosome instability, resulting in an unbalanced number of chromosomes in the daughter cells. Light microscopy has been used extensively to study chromosome missegregation by visualizing


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