Protocols shared by authors via RaP service
Journal
Enzyme-linked immunosorbent assay (ELISA)
Authors: Maria E. Diaz-Ortiz and Alice S. Chen-Plotkin
Date: Aug 30, 2022，Views：551
Original protocol used in research article：
GPNMB confers risk for Parkinson’s disease through interaction with α-synuclein
Science, Aug 19, 2022; DOI: 10.1126/science.abk0637
Immunofluorescence
Authors: Francesco M Piccolo and Ali H Brivanlou
Date: Aug 30, 2022，Views：549
Original protocol used in research article：
Role of YAP in early ectodermal specification and a Huntington's Disease model of human neurulation
eLife, ; DOI: 10.7554/eLife.73075
Quantitative RT-PCR
Authors: Zhihui Liu and Thomas C Südhof
Date: Aug 29, 2022，Views：597
Original protocol used in research article：
Deletion of Calsyntenin-3, an atypical cadherin, suppresses inhibitory synapses but increases excitatory parallel-fiber synapses in cerebellum
eLife, Apr 14, 2022; DOI: 10.7554/eLife.70664
Inference of copy number variations and clonality analysis.
Authors: Xiyuan Zhang and Angela C. Hirbe
Date: Aug 29, 2022，Views：798
Original protocol used in research article：
Chromosome 8 gain is associated with high-grade transformation in MPNST
JCI Insight, Mar 22, 2021; DOI: 10.1172/jci.insight.146351
Triglycerides and glycogen quantifications
Authors: Katarina Akhmetova and Igor Chesnokov
Date: Aug 29, 2022，Views：569
Original protocol used in research article：
Drosophila STING protein has a role in lipid metabolism
eLife, Sep 1, 2021; DOI: 10.7554/eLife.67358
Immunostaining
Authors: Amanda C Ignacz and Clarissa A Henry
Date: Aug 29, 2022，Views：579
Original protocol used in research article：
Beneficial impacts of neuromuscular electrical stimulation on muscle structure and function in the zebrafish model of Duchenne muscular dystrophy
eLife, ; DOI: 10.7554/eLife.62760
Luciferase assay
Authors: Jochen Winterer and Gerhard Schratt
Date: Aug 29, 2022，Views：678
Original protocol used in research article：
MicroRNA-138 controls hippocampal interneuron function and short-term memory in mice
eLife, Mar 15, 2022; DOI: 10.7554/eLife.74056
Genotyping dHT mice and real-time qPCR
Authors: Francesco Zorzato and Alexis Ruiz
Date: Aug 29, 2022，Views：578
Original protocol used in research article：
Improvement of muscle strength in a mouse model for congenital myopathy treated with HDAC and DNA methyltransferase inhibitors
eLife, Mar 3, 2022; DOI: 10.7554/eLife.73718
2.3.1. The Cognitive Impulsivity Suite (CIS)
Authors: Katharina Voigt and Antonio Verdejo-Garcia
Date: Aug 28, 2022，Views：630
Original protocol used in research article：
The Hunger Games: Homeostatic State-Dependent Fluctuations in Disinhibition Measured with a Novel Gamified Test Battery
Nutrients, Jun 10, 2021; DOI: 10.3390/nu13062001
Flow cytometry
Authors: Shuang Wang, Wei Wei and Guanghui Ma
Date: Aug 26, 2022，Views：544
Original protocol used in research article：
Macrophage-tumor chimeric exosomes accumulate in lymph node and tumor to activate the immune response and the tumor microenvironment
Science Translational Medicine, Oct 13, 2021; DOI: 10.1126/scitranslmed.abb6981
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