I received my PhD in Pharmaceutics in 2015, during which I developed a nanotechnology-based system to deliver galantamine to the brain intranasally to manage Alzheimer's disease. I have become fascinated by the blood-brain barrier functionality ever since, trying to understand how it is dynamically altered in pathophysiological statuses and capitalizing on this knowledge to design treatments far more efficiently.

As a senior postdoc at the University Hospital Bonn, I have been working with a novel blood-brain barrier model based on acute murine brain slices and real-time two photon microscopic imaging. I have been using this model to study the blood-brain barrier dynamics in different diseases such as epilepsy and Alzheimer’s disease and to identify the developmental

I am a molecular biologist by training and interested in studying virulence factors of Shigella – a Gram negative bacterial pathogen. I learnt about Bio-protocol when I started as a Postdoc and my PI asked me to submit a protocol that I had recently been working on. It was a great exercise as not only do I keep referring back to that protocol, but it was also featured as one of the most viewed protocols that year. The fine details mentioned in each bio-protocol is one of the key features that ensures reproducibility. As an ambassador, I wish to promote Bio-protocol and inspire other researchers to submit their optimized protocols in order to make best of their experience.

I am a final year PhD student in quite a small laboratory. My project concentrates on screening immortalised cancer cell lines with different biological macromolecules. I have found that I spend a lot of time comparing methods from different sources when establishing protocols for my own experiments. Often, there are major differences between sources which leads to confusion and difficulties when reproducing experiments.

By being a Bio-protocol's Ambassador, I hope I will be able to standardise experimental protocols which can then be optimised for specific laboratory procedures. This will reduce the time spent trying to create new protocols and, given the open accessibility of Bio-protocol, enable researchers to generate reproducible data that can be easily traced.

I am a science wanderer, enthusiastic enough to lead my research journey in the field of molecular cell biology, bioinformatics, marine biotechnology, and clinical microbiology. Currently, as Post-doc at UiT The Arctic University of Norway, I am involved in developing methods for investigating phenotypic heterogeneity of bacteria at single-cell resolution. Since single-cell study is my new play, I love working with microfluidics, artificial cells, and probe chemistry. My method-developing passion encourages me to learn and adapt to new methods and protocols. Thus, Bio-protocol is my platform of choice for learning and popularizing novel methods that I am working with. My upcoming teacher's training program also encourages me to let students know more about the science reproducibility and ethical perspective in

I'm a postdoctoral fellow working in the field of molecular neurosciences at Stanford University. I always aspire to become a part of initiatives that promote activities that are imperative to mend the problems that afflict today’s science. I believe open science is better science and envisage an utopian scientific enterprise where everything is transparent, reproducible, open to all and ethically done. I truly believe it is possible to push for such changes in this age, where the resources are endless. In my previous role as an early career researcher Ambassador with eLife, I was actively involved in promoting the need for reproducibility and transparency in bioscience. I’ve gained valuable insights of science communication, integrity, ethics and philosophy during my PhD and postdoctoral trainings at

A fun fact about me is that I wanted to marry a penguin when I was a kid. That's how enthusiastic I was about nature and wildlife. Hi, this is Mariam Elbaz, a 22 year old Egyptian woman. I've just graduated from the Faculty of Science, Ain shams University. I did a Bachelor degree in Biotechnology. During my study, I noticed the drastic disconnect between science and society's understanding of it. That's when I knew I wanted to fill this gap. Aside from marrying a penguin, I wanted to become a writer, a storyteller, specifically a visual storyteller. That's why I am interested in science communication. I want to help people contemplate the beauty of nature and help scientists communicate their work to the public. Nice to meet you, fellow human.

Mary is an exploration enthusiast. She has multidisciplinary and intercontinental experience. Her research philosophy is using molecular techniques to contribute to sustainable agriculture. As a Ph.D. student, her current heartache is the omission of detailed research methodologies in published papers. This poses a significant impediment to research reproducibility and effective science communication.

Being a Bio-protocol ambassador, she wants to contribute to making life easier for trainees like herself. Ultimately, her contribution will support the creation of a database for methodologies that could be easily cited. This will enable researchers to focus on using word limit restrictions to showcase their exciting results. To achieve this, she intends to leverage her diverse

Auxiliary researcher at ITQB NOVA in plant-microbes beneficial interactions. I'm focused in stress-tolerance enhancement by using approaches as epigenetic regulation, multi-omics and signalling-involved molecules characterization and modelling.

Wantao Noah Xiao is currently a PhD student in Biotechnology program at Brock University after completing his Master of Science degree at McMaster University. His research projects integrate many aspects of STEM research, ranging from plant biology to neuroscience. For that reason, Noah enjoys learning a variety of techniques and skills in the lab. His biggest hobby is researching everyday common gadgets and learning the mechanism of their function.

I am a 4th year PhD student at UT Southwestern Medical Center studying the role of the gut microbiota in the modulation of cancer immune checkpoint inhibitor therapy. I am from El Paso, TX and attended UT El Paso for my undergraduate degree in Microbiology. I am Hispanic (Mexican-American) and I speak English and Spanish. I am excited to be a Bio-protocol Ambassador because I am passionate about inclusive science communication. I hope to be able to share with my community how important it is to increase research reproducibility and network with like-minded scientists around the world.

During my graduate research career I experienced several challenges in having reproducibility with experimental data. Bio-protocol has always been one such reliable resource since my formative years of research. As a Bio-protocol Ambassador I want to address the challenges faced by scientists and help share the vision of Bio-protocol with the scientific community world-wide to improve reproducibility, and make sure their scientific journey is as productive. Science enables us to discover and better understand some of the challenges faced by humanity,and thus allows us to promote, create effective solutions to overcome them. The innovation and rapidly evolving technology offers us the best platforms to perform scientific experiments. However, scientists rely on highly-efficient methodologies to test their hypothesis. Based on my background in molecular biology, biochemistry and applications of some analytical tools, I strongly believe, precision in an experimental protocol remains primary, as they contain all the key elements for reproducibility and accuracy. With the plethora of information coming our way, it can be overwhelming and stressful while performing scientific research. It is imperative to have easily accessible platforms that not just provide accurate information but simplify techniques. I believe in creating an interactive platform for engaging talents from various diverse backgrounds that help us provide and exchange knowledge and enhance our learning in creating a better world.

Temitayo is an accomplished science communicator and a protean writer with a wealth of experience writing for blogs, magazines, other periodicals, and start-ups. He holds a bachelor's degree in microbiology and is on the verge of obtaining his PhD in Microbiology and Immunology. Tayo has won several notable awards for his science communication skills, and he is currently the communications chair of the graduate school at Louisiana State University Health Sciences Center Shreveport. When he is not pipetting or bridging the gap between scientists and the public, Tayo enjoys watching and playing soccer.

I am grateful for the opportunity to be a part of the Ambassador program. Bio-protocol is one of my favorite journals because I am convinced that experimental reproducibility is critical for research quality. One of my primary research objectives is to develop new methods and optimize or adapt existing approaches to studying new questions, with a focus on reproducibility and accuracy. To increase their chances of being useful to a large research community, I want to develop new approaches and techniques that are reliable, accurate, and cost-effective. That is why I support Bio-protocol's mission of promoting experimental reproducibility by publishing systematic detailed experimental procedures that can be used even by people who are new to a particular field.

I am a PhD student in Chemistry and Biochemistry at Ohio University. I am very passionate about learning new things in order to broaden my vision and improve current research methods. I believe it is very important to establish a reproducible and transparent science environment. I am fascinated about the idea of building an open-access, reproducible platform through Bio-protocol to benefit more scientists. I have been active in volunteering in different organizations for event support and advertising. I would like to help Bio-protocol build a better platform for scientists who can exchange ideas and stories to enhance the scientific community.

I very much appreciate the initiative to create this Ambassador program to promote experimental reproducibility and to highlight Bio-protocol to the broad scientific community. I am advocating for open and transparent science through several programs and enjoy promoting the exchange of ideas and thoughts within the early career researchers’ community. As an author, reviewer, and associate editor, I am well aware of the mission, objectives, and team efforts Bio-protocol in the promotion experimental reproducibility by publishing step-by-step detailed experimental procedures which can be used even by first hand undergraduate students. I would like to promote this platform, through the University where I have direct contact with undergraduates through Bachelor/Master Courses.

It's striking how many general lab protocols lack clarity and require interpretation. Most common protocols are shared in an ongoing game of telephone, many of which are clearly designed by very clever people without enough time or attention to ensure that they are unambiguous for inexperienced users. I appreciate the dedicated position that Bio-protocols takes on the matter, that reproducibility and openness are as critical as the data. I haven’t been in science for too long, but I really enjoy tinkering with and improving protocols, and want to support the Bio-protocol mission of peer-reviewed publications of efficient and clear protocols.

As a "methods-centric" research group, our livelihood depends upon the robustness and reproducibility of our work. We routinely publish in methods journals and edited books, including open access ones. We are partly funded under a 'Focused Technology Research and Development' mechanism from NIGMS – thus, we are charged by the NIH with producing and disseminating reliable methods.

I want to use my opportunity as Bio-protocol ambassador to enhance how I can spread, what I believe is, a realistic and humble view of the investigator / open science relationship. I firmly believe that Bio-protocol is an important platform in the cultural shift occurring in research dissemination.

In my tenure as a researcher for more than 12 years, there have been a few incidences where I tried to reproduce data published in leading research journals and failed. To overcome these hurdles, it is imperative that scientists document and share exact experimental procedures. I have been reviewing papers for Bio-protocol and following research articles submitted there. Reading step by step protocols and following them for an experiment has made it easier for me to reproduce experiments. I am a firm believer of open access to science by open science or preprints will help science be more reproducible.