Protocols shared by authors via RaP service
Journal
Exogenous ecdysone feeding treatments
Authors: Alexander Shingleton and Christen K Mirth
Date: Aug 1, 2022，Views：675
Original protocol used in research article：
Ecdysone coordinates plastic growth with robust pattern in the developing wing
eLife, Mar 9, 2022; DOI: 10.7554/eLife.72666
3.10. Flow Cytometry-Based Proliferation Assays
Authors: Jiayao Fu, Chuangqi Yu and Lingyan Zheng
Date: Aug 1, 2022，Views：573
Original protocol used in research article：
Pharmacological Inhibition of Glutaminase 1 Normalized the Metabolic State and CD4+ T Cell Response in Sjogren's Syndrome
Journal of Immunology Research, Feb 15, 2022; DOI: 10.1155/2022/3210200
ELISA and real-time PCR
Authors: Gilad Barshad, Elia D. Tait Wojno and Lauren Webb
Date: Aug 1, 2022，Views：567
Original protocol used in research article：
E-Protein Inhibition in ILC2 Development Shapes the Function of Mature ILC2s during Allergic Airway Inflammation
J Immunol, Mar 1, 2022; DOI: 10.4049/jimmunol.2100414
Preparation of pancreatic duct cells for single-cell analysis
Authors: Audrey M Hendley and Matthias Hebrok
Date: Jul 31, 2022，Views：587
Original protocol used in research article：
Single-cell transcriptome analysis defines heterogeneity of the murine pancreatic ductal tree
eLife, May 19, 2021; DOI: 10.7554/eLife.67776
Whole mount in situ hybridization
Authors: Elena Vasileva and James F Amatruda
Date: Jul 29, 2022，Views：570
Original protocol used in research article：
Dysregulated heparan sulfate proteoglycan metabolism promotes Ewing sarcoma tumor growth
eLife, Mar 14, 2022; DOI: 10.7554/eLife.69734
Cell line transfection
Authors: Giacomo Frati and Annarita Miccio
Date: Jul 29, 2022，Views：585
Original protocol used in research article：
Editing a γ-globin repressor binding site restores fetal hemoglobin synthesis and corrects the sickle cell disease phenotype
Science Advances, Feb 12, 2020; DOI: 10.1126/sciadv.aay9392
Hematoxylin & Eosin (H&E) staining
Authors: Hyun-Jun Kim, Da-Woon Jung and Darren R. Williams
Date: Jul 29, 2022，Views：668
Original protocol used in research article：
Investigation of niclosamide as a repurposing agent for skeletal muscle atrophy
PLoS ONE, May 26, 2021; DOI: 10.1371/journal.pone.0252135
4.4. Genome annotation
Authors: Rui-Fang Gao, Chuan-Ming Yeh, Kazutaka Katoh, Wen-Chieh Tsai, Zhong-Jian Liu, Francis Martin and Gui-Ming Zhang
Date: Jul 28, 2022，Views：546
Original protocol used in research article：
Comparative analysis of Phytophthora genomes reveals oomycete pathogenesis in crops
Heliyon, Feb 23, 2021; DOI: 10.1016/j.heliyon.2021.e06317
Isolation of RNA from the murine colonic tissue and qRT-PCR for inflammatory cytokines
Authors: Ibrahim M. Sayed, Kaili M Inouye, Soumita Das and Laura E Crotty Alexander
Date: Jul 28, 2022，Views：691
Original protocol used in research article：
Effects of mango and mint pod-based e-cigarette aerosol inhalation on inflammatory states of the brain, lung, heart, and colon in mice
eLife, Apr 12, 2022; DOI: 10.7554/eLife.67621
Isolation and characterization of peritoneal immune cells
Authors: Hu Li, Jan M. Van Deursen and Darren Baker
Date: Jul 27, 2022，Views：582
Original protocol used in research article：
p21 produces a bioactive secretome that places stressed cells under immunosurveillance
Science, Oct 29, 2021; DOI: 10.1126/science.abb3420
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