Bio-101 - Improve Research Reproducibility

Isolation of Tonsil B Cells

Authors: Magalie Michée-Cospolite, Laetitia Le Pottier and Sophie Hillion
Date: Jul 22, 2022，Views：546

Original protocol used in research article：

Molecular Mechanisms Driving IL-10- Producing B Cells Functions: STAT3 and c-MAF as Underestimated Central Key Regulators?
Frontiers in Immunology, Mar 10, 2022; DOI: 10.3389/fimmu.2022.818814

Fusion primer and nested integrated PCR (FPNI-PCR)

Author: Shengming Yang
Date: Jul 22, 2022，Views：642

Original protocol used in research article：

Genetic analysis of the barley variegation mutant, grandpa1.a
BMC Plant Biology, Mar 13, 2021; DOI: 10.1186/s12870-021-02915-9

IP accumulation assay

Authors: Anette Kaiser, Annette G. Beck-Sickinger, Qiang Zhao and Beili Wu
Date: Jul 22, 2022，Views：684

Original protocol used in research article：

Receptor-specific recognition of NPY peptides revealed by structures of NPY receptors
Science Advances, May 4, 2022; DOI: 10.1126/sciadv.abm1232

Materials_Methods

Authors: Filippo Birocchi, Nadia Coltella and Luigi Naldini
Date: Jul 22, 2022，Views：567

Original protocol used in research article：

Targeted inducible delivery of immunoactivating cytokines reprograms glioblastoma microenvironment and inhibits growth in mouse models
Science Translational Medicine, Jul 13, 2022; DOI: 10.1126/scitranslmed.abl4106

2.4. Incubation in Simulated Body Fluid (SBF)

Author: Michael Seidenstuecker
Date: Jul 22, 2022，Views：598

Original protocol used in research article：

Inverse 3D Printing with Variations of the Strand Width of the Resulting Scaffolds for Bone Replacement
Materials, Apr 14, 2021; DOI: 10.3390/ma14081964

Staining of cytospin preparations of AM populations

Authors: Federica Piattini and Manfred Kopf
Date: Jul 19, 2022，Views：574

Original protocol used in research article：

Monocyte-derived alveolar macrophages autonomously determine severe outcome of respiratory viral infection
Science Immunology, Jul 1, 2022; DOI: 10.1126/sciimmunol.abj5761

RNA isolation, RT-PCR, and library preparation

Authors: Alex Stemm-Wolf and Chad G. Pearson
Date: Jul 19, 2022，Views：572

Original protocol used in research article：

The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis
Molecular Biology of the Cell, Sep 9, 2021; DOI: 10.1091/mbc.E21-06-0305

4.4. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) Determination by Broth Microdilution and Resazurin Colorimetric Assay

Authors: Oranee Srichaiyapol and Rina Patramanon
Date: Jul 18, 2022，Views：707

Original protocol used in research article：

Tannic Acid-Stabilized Silver Nanoparticles Used in Biomedical Application as an Effective Antimelioidosis and Prolonged Efflux Pump Inhibitor against Melioidosis Causative Pathogen
Molecules, Feb 14, 2021; DOI: 10.3390/molecules26041004

Novel environment test and novel object test

Author: Allyson F. Ipema
Date: Jul 18, 2022，Views：551

Original protocol used in research article：

Providing live black soldier fly larvae (Hermetia illucens) improves welfare while maintaining performance of piglets post-weaning
Scientific Reports, Apr 1, 2021; DOI: 10.1038/s41598-021-86765-3

Flow cytometry of PBMCs

Authors: Heather M. Gibson, Justin B. Hackett and Claire McCarthy-Leo
Date: Jul 18, 2022，Views：573

Original protocol used in research article：

A diversity outbred F1 mouse model identifies host-intrinsic genetic regulators of response to immune checkpoint inhibitors
Oncoimmunology, Apr 20, 2022; DOI: 10.1080/2162402X.2022.2064958

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