Protocols shared by authors via RaP service
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2.5.4. Cupric Ion Reducing Antioxidant Capacity (CUPRAC) Assay
Authors: Ahmed G. Darwish, Protiva Rani Das and Islam El-Sharkawy
Date: Jul 17, 2022，Views：573
Original protocol used in research article：
Untargeted Metabolomics and Antioxidant Capacities of Muscadine Grape Genotypes during Berry Development
Antioxidants, Jun 4, 2021; DOI: 10.3390/antiox10060914
Untargeted and targeted lipidomics – statistical analysis: Factoria ANOVA in R
Authors: Lucas Carminatti Pantaleao, Isabella Inzani and Susan E Ozanne
Date: Jul 15, 2022，Views：572
Original protocol used in research article：
Maternal diet-induced obesity during pregnancy alters lipid supply to mouse E18.5 fetuses and changes the cardiac tissue lipidome in a sex-dependent manner
eLife, Jan 13, 2022; DOI: 10.7554/eLife.69078
preparation of nanoparticles by sonication
Authors: Zhao Zhang, Vincent Terrasson and Erwann Guénin
Date: Jul 15, 2022，Views：545
Original protocol used in research article：
Lignin Nanoparticles and Their Nanocomposites
Nanomaterials, May 19, 2021; DOI: 10.3390/nano11051336
Preparation of cell extracts
Authors: Toshiaki Tanaka and Koji Moriya
Date: Jul 15, 2022，Views：558
Original protocol used in research article：
Visualized procollagen Iα1 demonstrates the intracellular processing of propeptides
Life Science Alliance, Feb 18, 2022; DOI: 10.26508/lsa.202101060
Reverse transcription PCR and quantitative PCR analysis
Author: Sung Wook Seo
Date: Jul 15, 2022，Views：585
Original protocol used in research article：
GNAQ knockdown promotes bone metastasis through epithelial-mesenchymal transition in lung cancer cells
Bone & Joint Research, May 17, 2021; DOI: 10.1302/2046-3758.105.BJR-2020-0262.R3
Dissociation of adult rodent DRG for single-cell RNAseq
Authors: Oshri Avraham and Valeria Cavalli
Date: Jul 14, 2022，Views：647
Original protocol used in research article：
Profiling sensory neuron microenvironment after peripheral and central axon injury reveals key pathways for neural repair
eLife, Sep 29, 2021; DOI: 10.7554/eLife.68457
Protein extraction and ELISA
Author: Idit Sagiv Barfi
Date: Jul 13, 2022，Views：624
Original protocol used in research article：
Intratumoral immunotherapy relies on B and T cell collaboration
Science Immunology, May 27, 2022; DOI: 10.1126/sciimmunol.abn5859
HLA Typing by High-Resolution Next-Generation Sequencing
Authors: Roos A. W. Wennink and Jonas J. W. Kuiper
Date: Jul 13, 2022，Views：567
Original protocol used in research article：
Next-Generation HLA Sequence Analysis Uncovers Shared Risk Alleles Between Clinically Distinct Forms of Childhood Uveitis
Investigative Ophthalmology & Visual Science, Jul 13, 2021; DOI: 10.1167/iovs.62.9.19
Cell-to-cell fusion assay
Authors: Tarun Mishra, Rishikesh Dalavi and Ajit Chande
Date: Jul 13, 2022，Views：1131
Original protocol used in research article：
SARS-CoV-2 spike E156G/Δ157-158 mutations contribute to increased infectivity and immune escape
Life Science Alliance, Mar 16, 2022; DOI: 10.26508/lsa.202201415
ChR2-assisted circuit mapping
Author: Ji Liu
Date: Jul 13, 2022，Views：576
Original protocol used in research article：
Glucose-sensing glucagon-like peptide-1 receptor neurons in the dorsomedial hypothalamus regulate glucose metabolism
Science Advances, Jun 8, 2022; DOI: 10.1126/sciadv.abn5345
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