Plant Science


    Agrobacterium-mediated Genetic Transformation of Cotton and Regeneration via Somatic Embryogenesis

    Cotton is a significant industrial crop, playing an essential role in the global economy that suffers several setbacks due to biotic and abiotic adversities. Despite such problems, biotechnological advances in cotton are limited because of genetic

    Metagenomic Protocol (From Quality Control to Mapping) for Metagenome-assembled Genomes Using Anvi’o
    Authors:  Soumyadev Sarkar, Tanner Richie and Sonny T. M. Lee, date: 05/20/2023, view: 40, Q&A: 0

    Synapses provide the main route of signal transduction within neuronal networks. Many factors regulate critical synaptic functions. These include presynaptic calcium channels, triggering neurotransmitter release, and postsynaptic ionotropic

    Visualizing, Binning, and Refining of Metagenome-assembled Genomes (MAGs) with Anvi’o
    Authors:  Soumyadev Sarkar, Tanner Richie and Sonny T. M. Lee, date: 05/20/2023, view: 20, Q&A: 0

    High throughput ‘omics technologies generate huge datasets that need to be properly analyzed in order to decipher the biological implications. The workflow of handling such datasets must be user friendly to facilitate rapid analysis. Here, we

    Modified Pseudo-Schiff Propidium Iodide for Staining the Shoot Apical Meristem in Arabidopsis
    Authors:  Ruiqi Li, Qi Li and Ligeng Ma, date: 05/05/2023, view: 279, Q&A: 0

    Visualization of cell structure with fluorescent dye for characterizing cell size, shape, and arrangement is a common method to study tissue morphology and morphogenesis. In order to observe shoot apical meristem (SAM) in Arabidopsis thaliana by

    Synthetic Promoter Screening Using Poplar Mesophyll Protoplast Transformation

    Plant protoplasts are useful to study both transcriptional regulation and protein subcellular localization in rapid screens. Protoplast transformation can be used in automated platforms for design-build-test cycles of plant promoters, including

    Determination of Paraquat in Arabidopsis Tissues and Protoplasts by UHPLC-MS/MS
    Authors:  Mingming Zhao, Qi Wang, Muyu Shi, Ziyan Sun, Huiru Tang and Xiaochun Ge, date: 04/05/2023, view: 317, Q&A: 0

    Paraquat is a cost-effective herbicide, widely used in many countries, that can induce severe oxidative stress in photosynthetic tissues. Studying plant herbicide resistance or antioxidant stress mechanisms requires determining the cellular paraquat

    Rapid and Reliable Quantification of Glycerol-3-phosphate Using Gas Chromatography–coupled Mass Spectrometry
    Authors:  Huazhen Liu, Keshun Yu and Pradeep Kachroo, date: 04/05/2023, view: 287, Q&A: 0

    Glycerol-3-phosphate (G3P) is a conserved precursor of glycerolipids that also plays an important role in plant defense. Its levels and/or metabolism are also associated with many human disorders including insulin resistance, diabetes, obesity, and

    Quantification of Ethylene Production in Leaf and Bud Tissue of the Subtropical Tree Crop Litchi (Litchi chinensis Sonn.) Using Gas Chromatography and Flame Ionization Detection
    Authors:  Regina B. Cronje and Arnoldus J. Jonker, date: 03/20/2023, view: 272, Q&A: 0

    Ethylene is an important plant hormone that is involved in the regulation of numerous processes in plant development. It also acts as a signaling molecule in response to biotic and abiotic stress conditions. Most studies have investigated ethylene

    A Miniature Sucrose Gradient for Polysome Profiling
    Authors:  Ansul Lokdarshi and Albrecht G. von Arnim, date: 03/20/2023, view: 510, Q&A: 0

    Polysome profiling by sucrose density gradient centrifugation is commonly used to study the overall degree of translation (messenger RNA to protein synthesis). Traditionally, the method begins with synthesis of a 5–10 mL sucrose gradient onto

    Isolation of Intact Vacuoles from Arabidopsis Root Protoplasts and Elemental Analysis
    Authors:  Chuanfeng Ju, Dali Fu, Cun Wang and Zhenqian Zhang, date: 03/05/2023, view: 219, Q&A: 0

    The vacuole is one of the most conspicuous organelles in plant cells, participating in a series of physiological processes, such as storage of ions and compartmentalization of heavy metals. Isolation of intact vacuoles and elemental analysis


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