Developmental Biology


    An Improved Protocol for the Matrigel Duplex Assay: A Method to Measure Retinal Angiogenesis

    Neovascular diseases of the retina, such as diabetic retinopathy (DR) and age-related macular degeneration (AMD), are proliferative retinopathies involving the growth of new blood vessels on the retina, which in turn causes impairment and potential

    A Methodology for the Enzymatic Isolation of Embryonic Hypothalamus Tissue and Its Acute or Post-Culture Analysis by Multiplex Hybridisation Chain Reaction
    Authors:  Kavitha Chinnaiya and Marysia Placzek, date: 12/05/2023, view: 46, Q&A: 0

    The hypothalamus is an evolutionarily ancient part of the vertebrate ventral forebrain that integrates the dialogue between environment, peripheral body, and brain to centrally govern an array of physiologies and behaviours. Characterizing the

    Preparation of Cardiac Extracts from Embryonal Hearts to Capture RNA–protein Interactions by CLIP

    The interaction of RNA with specific RNA-binding proteins (RBP) leads to the establishment of complex regulatory networks through which gene expression is controlled. Careful consideration should be given to the exact environment where a given

    A Rapid and Simple Procedure for the Isolation of Embryonic Cells from Fish Eggs

    Fertilized teleost fish eggs are a complex formation, in which dividing cells arelocated in a small point in the entire volume of eggs. Isolating embryonic cellscan be considered a necessary step in the research of developmentalpeculiarities of fish

    Spatial Centrosome Proteomic Profiling of Human iPSC-derived Neural Cells
    Authors:  Fatma Uzbas and Adam C. O’Neill, date: 09/05/2023, view: 443, Q&A: 0

    The centrosome governs many pan-cellular processes including cell division, migration, and cilium formation. However, very little is known about its cell type-specific protein composition and the sub-organellar domains where these protein

    Isolation of Embryonic Cardiomyocytes and Cell Proliferation Assay Using Genetically Engineered Reporter Mouse Model
    Authors:  Maren Beall, Deqiang Li and Jihyun Jang, date: 09/05/2023, view: 464, Q&A: 0

    Congenital heart disease (CHD) is often associated with myogenic defects. During heart development, cardiomyocyte growth requires essential cues from extrinsic factors such as insulin-like growth factor 2 (IGF-2). To determine whether and how growth

    Stereotactic Delivery of Helper-dependent Adenoviral Viral Vectors at Distinct Developmental Time Points to Perform Age-dependent Molecular Manipulations of the Mouse Calyx of Held
    Authors:  Christian Keine, Mohammed Al-Yaari, Tamara Radulovic and Samuel M. Young Jr., date: 08/20/2023, view: 399, Q&A: 0

    Synapses are specialized structures that enable neuronal communication, which is essential for brain function and development. Alterations in synaptic proteins have been linked to various neurological and neuropsychiatric disorders. Therefore,

    Fabrication of Microfluidic Devices for Continuously Monitoring Yeast Aging
    Authors:  Richard O’Laughlin, Emerald Forrest, Jeff Hasty and Nan Hao, date: 08/05/2023, view: 268, Q&A: 0

    For several decades, aging in Saccharomyces cerevisiae has been studied in hopes of understanding its causes and identifying conserved pathways that also drive aging in multicellular eukaryotes. While the short lifespan and unicellular nature of

    Fluorescent PCR–based Screening Methods for Precise Knock-in of Small DNA Fragments and Point Mutations in Zebrafish
    Authors:  Blake Carrington and Raman Sood, date: 08/05/2023, view: 329, Q&A: 0

    Generation of zebrafish (Danio rerio) models with targeted insertion of epitope tags and point mutations is highly desirable for functional genomics and disease modeling studies. Currently, CRISPR/Cas9-mediated knock-in is the method of choice for

    Development of a Mouse Model of Hematopoietic Loss of Y Chromosome
    Authors:  Soichi Sano and Kenneth Walsh, date: 08/05/2023, view: 287, Q&A: 0

    This protocol describes the generation of chimeric mice in which the Y chromosome is deleted from a proportion of blood cells. This model recapitulates the phenomenon of hematopoietic mosaic loss of Y chromosome (mLOY), which is frequently observed


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