Developmental Biology

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    In vivo Characterization of Endogenous Protein Interactomes in Drosophila Larval Brain, Using a CRISPR/Cas9-based Strategy and BioID-based Proximity Labeling
    Authors:  Ezgi Uçkun, Georg Wolfstetter, Johannes Fuchs and Ruth H. Palmer, date: 07/05/2022, view: 22, Q&A: 0

    Understanding protein-protein interactions (PPIs) and interactome networks is essential to reveal molecular mechanisms mediating various cellular processes. The most common method to study PPIs in vivo is affinity purification combined with mass

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    ATAC-Seq of a Single Myofiber from Mus musculus
    Authors:  Korin Sahinyan, Darren M. Blackburn and Vahab D. Soleimani, date: 06/20/2022, view: 1225, Q&A: 0

    Chromatin accessibility is a key determinant of gene expression that can be altered under different physiological and disease conditions. Skeletal muscle is made up of myofibers that are highly plastic and adaptive. Therefore, assessing the

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    Quantitative Live Confocal Imaging in Aquilegia Floral Meristems
    Authors:  Ya Min, Stephanie J. Conway and Elena M. Kramer, date: 06/20/2022, view: 992, Q&A: 0

    In this study, we present a detailed protocol for live imaging and quantitative analysis of floral meristem development in Aquilegia coerulea, a member of the buttercup family (Ranunculaceae). Using confocal microscopy and the image analysis

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    Labelling of Active Transcription Sites with Argonaute NRDE-3—Image Active Transcription Sites in vivo in Caenorhabditis elegans
    Authors:  Antoine Barrière and Vincent Bertrand, date: 06/05/2022, view: 519, Q&A: 0

    Live labelling of active transcription sites is critical to our understanding of transcriptional dynamics. In the most widely used method, RNA sequence MS2 repeats are added to the transcript of interest, on which fluorescently tagged Major Coat

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    Electroporation of Small Interfering RNAs into Tibialis Anterior Muscles of Mice
    Authors:  Anna Stephan, Flavia A. Graca, Liam C. Hunt and Fabio Demontis, date: 06/05/2022, view: 787, Q&A: 0

    Aging and wasting of skeletal muscle reduce organismal fitness. Regrettably, only limited interventions are currently available to address this unmet medical need. Many methods have been developed to study this condition, including the

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    Simple Methods for Permanent or Transient Denervation in Mouse Sciatic Nerve Injury Models

    Our ability to move and breathe requires an efficient communication between nerve and muscle that mainly takes place at the neuromuscular junctions (NMJs), a highly specialized synapse that links the axon of a motor neuron to a muscle fiber. When

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    Click-iT® Plus OPP Alexa Fluor® Protein Synthesis Assay in Embryonic Cells

    This protocol describes a method to assess relative changes in the level of global protein synthesis in the preimplantation embryo using the Click-iT® Plus OPP Protein Synthesis Assays. In this assay, O-propargyl-puromycin (OPP), an analog of

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    Efficient Superovulation and Egg Collection from Mice
    Authors:  Miyuki Shindo, Kenji Miyado, Woojin Kang, Maki Fukami and Mami Miyado, date: 06/05/2022, view: 555, Q&A: 0

    Superovulation is a method used to reduce the number of mice used per experiment by increasing the egg number. Conventionally, superovulation for obtaining mouse eggs involves the use of equine chorionic gonadotropin (eCG) for stimulation and human

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    Preparation of a Single Cell Suspension from the Murine Iridocorneal Angle
    Authors:  Benjamin R. Thomson and Susan E. Quaggin, date: 05/20/2022, view: 591, Q&A: 0

    Single cell RNA sequencing is a powerful tool that can be used to identify distinct cell types and transcriptomic differences within complex tissues. It has proven to be especially useful in tissues of the eye, where investigators have identified

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    Isolation of tdTomato Expressing Inter-follicular Epidermal Melanocytes or Keratinocytes from Mouse Tail Skin
    Authors:  Samuel Pop, Oscar Urtatiz and Catherine D. Van Raamsdonk, date: 04/20/2022, view: 1113, Q&A: 0

    The epidermis is the outermost layer of the skin. It is made up of mostly keratinocytes along with a small number of melanocytes and Langerhans cells. Melanocytes produce a pigment called melanin, which is transferred to the keratinocytes, and

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