Biophysics

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    Patterned Substrate of Mobile and Immobile Ligands to Probe EphA2 Receptor Clustering
    Authors:  Zhongwen Chen, Kabir H. Biswas and Jay T. Groves, date: 06/05/2022, view: 779, Q&A: 0

    A multitude of membrane-localized receptors are utilized by cells to integrate both biochemical and physical signals from their microenvironment. The clustering of membrane receptors is widely presumed to have functional consequences for subsequent

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    Purification of Mitochondrial Ribosomal Complexes from Trypanosoma cruzi and Leishmania tarentolae for Cryo-EM Analysis
    Authors:  Stéphanie Durrieu-Gaillard, Marie Sissler and Yaser Hashem, date: 05/20/2022, view: 917, Q&A: 1

    Kinetoplastids are unicellular eukaryotic parasites responsible for human pathologies such as Chagas disease, sleeping sickness or Leishmaniasis, caused by Trypanosoma cruzi, Trypanosoma brucei, and various Leishmania spp., respectively. They harbor

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    Production and Crystallization of Nanobodies in Complex with the Receptor Binding Domain of the SARS-CoV-2 Spike Protein

    The receptor binding domain (RBD) of the spike protein of SARS-CoV-2 binds angiotensin converting enzyme-2 (ACE-2) on the surface of epithelial cells, leading to fusion, and entry of the virus into the cell. This interaction can be blocked by the

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    Quantitative Analysis of Actin Cable Length in Yeast
    Authors:  Shane G. McInally, Jane Kondev and Bruce L. Goode, date: 05/05/2022, view: 936, Q&A: 0

    Polarized actin cables in S. cerevisiae are linear bundles of crosslinked actin filaments that are assembled by two formins, Bnr1 (localized to the bud neck), and Bni1 (localized to the bud tip). Actin is polymerized at these two sites, which

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    Characterization of the Elasticity of CD4+ T Cells: An Approach Based on Peak Force Quantitative Nanomechanical Mapping
    Authors:  Philipp Jung, Xiangda Zhou, Sandra Iden, Bin Qu and Markus Bischoff, date: 04/20/2022, view: 906, Q&A: 0

    CD4+ T cells are essential players in orchestrating the specific immune response against intracellular pathogens, and in inhibiting tumor development in an early stage. The activation of T cells is triggered by engagement of T cell receptors (TCRs).

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    Bsoft: Image Processing for Structural Biology
    Author:  J. Bernard Heymann, date: 04/20/2022, view: 1194, Q&A: 0

    Bsoft is a software package primarily developed for processing electron micrographs, with the goal of determining the structures of biologically relevant molecules, molecular assemblies, and parts of cells. However, it incorporates many ways to deal

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    Single Molecule Tracking Nanoscopy Extended to Two Colors with MTT2col for the Analysis of Cell-Cell Interactions in Leukemia
    Authors:  Loriane Maillot, Magali Irla and Arnauld Sergé, date: 04/20/2022, view: 806, Q&A: 0

    Single molecule tracking (SMT) is a powerful technique to study molecular dynamics, and is particularly adapted to monitor the motion and interactions of cell membrane components. Assessing interactions among two molecular populations is classically

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    Activation of Mitochondrial Ca2+ Oscillation and Mitophagy Induction by Femtosecond Laser Photostimulation
    Authors:  Xiaoying Tian and Hao He, date: 04/05/2022, view: 446, Q&A: 0

    Ultra-precise stimulation solely to individual mitochondria, without any influence to the whole cell, is quite difficult by traditional biochemical reagents. In mitophagy research, the mitochondria and even the whole cell usually suffer

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    Quantitative and Anatomical Imaging of Human Skin by Noninvasive Photoacoustic Dermoscopy
    Authors:  Zhiyang Wang, Fei Yang, Wuyu Zhang and Sihua Yang, date: 04/05/2022, view: 506, Q&A: 0

    Imaging plays a vital role in the diagnosis and treatment of skin diseases. However, pure optical imaging technique is limited to the visualization of superficial skin tissues. Ultrasonic imaging technique can detect deep tissues, but it lacks

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    Electrophysiological Measurements of Isolated Blood Vessels
    Authors:  Samuel A Molina, Daniela Maier-Begandt, Brant E Isakson and Michael Koval, date: 03/20/2022, view: 591, Q&A: 0

    The lumen of blood vessels is covered by endothelial cells, which regulate their permeability to ions and solutes. Endothelial permeability depends on the vascular bed and cell phenotype, and is influenced by different disease states. Most

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