H2 Production from Methyl Viologen–Dependent Hydrogenase Activity Monitored by Gas Chromatography
    Author:  Nuttavut Kosem, date: 12/05/2023, view: 61, Q&A: 0

    Bio-hydrogen production is an eco-friendly alternative to commercial H2 production, taking advantage of natural systems. Microbial hydrogenases play a main role in biological mechanisms, catalyzing proton reduction to molecular hydrogen (H2)

    Purification of Human Cytoplasmic Actins From Saccharomyces cerevisiae
    Authors:  Brian K. Haarer, David C. Amberg and Jessica L. Henty-Ridilla, date: 12/05/2023, view: 34, Q&A: 0

    Eukaryotic cells rely on actin to support cellular structure, motility, transport, and a wide variety of other cytoplasmic functions and nuclear activities. Humans and other mammals express six closely related isoforms of actin, four of which are

    Expression and Purification of Recombinant Human Mitochondrial RNA Polymerase (POLRMT) and the Initiation Factors TFAM and TFB2M

    Human mitochondrial DNA (mtDNA) encodes several components of oxidative phosphorylation responsible for the bulk of cellular energy production. The mtDNA is transcribed by a dedicated human mitochondrial RNA polymerase (POLRMT) that is structurally

    Phospholipid Preparations to Characterize Protein–Lipid Interactions In Vitro
    Authors:  Lisa Merklinger and J. Preben Morth, date: 11/20/2023, view: 196, Q&A: 0

    The lipid bilayers of the cell are composed of various lipid classes and species. These engage in cell signaling and regulation by recruiting cytosolic proteins to the membrane and interacting with membrane-embedded proteins to alternate their

    A New, Rapid Method for the Quantification of Dolichyl Phosphates in Cell Cultures Using TMSD Methylation Combined with LC–MS Analysis
    Authors:  Dipali Kale, Timo Sachsenheimer, Albert Sickmann and Britta Brügger, date: 11/20/2023, view: 242, Q&A: 0

    Dolichyl phosphates (DolP) are ubiquitous lipids that are present in almost all eukaryotic membranes. They play a key role in several protein glycosylation pathways and the formation of glycosylphosphatidylinositol anchors. These lipids constitute

    Workflow for High-throughput Screening of Enzyme Mutant Libraries Using Matrix-assisted Laser Desorption/Ionization Mass Spectrometry Analysis of Escherichia coli Colonies
    Authors:  Kisurb Choe and Jonathan V. Sweedler, date: 11/05/2023, view: 279, Q&A: 0

    High-throughput molecular screening of microbial colonies and DNA libraries are critical procedures that enable applications such as directed evolution, functional genomics, microbial identification, and creation of engineered microbial strains to

    Biosynthesis and Genetic Encoding of Non-hydrolyzable Phosphoserine into Recombinant Proteins in Escherichia coli
    Authors:  Philip Zhu, Ryan A. Mehl and Richard B. Cooley, date: 11/05/2023, view: 278, Q&A: 0

    While site-specific translational encoding of phosphoserine (pSer) into proteins in Escherichia coli via genetic code expansion (GCE) technologies has transformed our ability to study phospho-protein structure and function, recombinant

    Fluorescence Resonance Energy Transfer to Detect Plasma Membrane Perturbations in Giant Plasma Membrane Vesicles
    Authors:  Mathew Sebastiao, Noé Quittot, Isabelle Marcotte and Steve Bourgault, date: 10/05/2023, view: 251, Q&A: 0

    Disruptions and perturbations of the cellular plasma membrane by peptides have garnered significant interest in the elucidation of biological phenomena. Typically, these complex processes are studied using liposomes as model membranes—either

    Protein Level Quantification Across Fluorescence-based Platforms
    Authors:  Hector Romero, Annika Schmidt and M. Cristina Cardoso, date: 10/05/2023, view: 523, Q&A: 0

    Biological processes are dependent on protein concentration and there is an inherent variability among cells even in environment-controlled conditions. Determining the amount of protein of interest in a cell is relevant to quantitatively relate it

    Identification of Matriglycan by Dual Exoglycosidase Digestion of α-Dystroglycan
    Authors:  Ishita Chandel and Kevin P. Campbell, date: 09/20/2023, view: 286, Q&A: 0

    Matriglycan is a linear polysaccharide of alternating xylose and glucuronic acid units [-Xyl-α1,3-GlcA-β1,3]n that is uniquely synthesized on α-dystroglycan (α-DG) and is essential for neuromuscular function and brain


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