Human Schwann Cells in vitro III. Analytical Methods and a Practical Approach for Quality Control
    Author:  Paula V. Monje, date: 10/05/2023, view: 28, Q&A: 0

    This paper introduces simple analytical methods and bioassays to promptly assess the identity and function of in vitro cultured human Schwann cells (hSCs). A systematic approach is proposed to unequivocally discriminate hSCs from other glial cells,

    Human Schwann Cells in vitro I. Nerve Tissue Processing, Pre-degeneration, Isolation, and Culturing of Primary Cells
    Authors:  Gabriela I. Aparicio and Paula V. Monje, date: 10/05/2023, view: 178, Q&A: 0

    This paper presents versatile protocols to prepare primary human Schwann cell (hSC) cultures from mature peripheral nervous system tissues, including fascicles from long spinal nerves, nerve roots, and ganglia. This protocol starts with a

    Spatial Centrosome Proteomic Profiling of Human iPSC-derived Neural Cells
    Authors:  Fatma Uzbas and Adam C. O’Neill, date: 09/05/2023, view: 249, Q&A: 0

    The centrosome governs many pan-cellular processes including cell division, migration, and cilium formation. However, very little is known about its cell type-specific protein composition and the sub-organellar domains where these protein

    Confocal Imaging and 3D Reconstruction to Determine How Genetic Perturbations Impact Presynaptic Morphology at the Mouse Calyx of Held
    Authors:  Christian Keine, Tamara Radulovic, Mohammed Al-Yaari and Samuel M. Young Jr., date: 09/05/2023, view: 480, Q&A: 0

    Neurons communicate via synapses—specialized structures that consist of a presynaptic terminal of one neuron and a postsynaptic terminal of another. As knowledge is emerging that mutations in molecules that regulate synaptic function underpin

    The Effects of Whole-body Cold-water Immersion on Brain Connectivity Related to the Affective State in Adults Using fMRI: A Protocol of a Pre-post Experimental Design
    Authors:  Ala Yankouskaya, Heather Massey, John James Totman, Lin Hui Lai and Ruth Williamson, date: 09/05/2023, view: 126, Q&A: 0

    An emerging body of behavioural studies indicates that regular swimming in cold water has positive effects on mental health and wellbeing, such as reducing fatigue, improving mood, and lessening depressive symptoms. Moreover, some studies reported

    A Method for Studying Social Signal Learning of the Waggle Dance in Honey Bees
    Authors:  Shihao Dong, Tao Lin, James C. Nieh and Ken Tan, date: 08/20/2023, view: 166, Q&A: 0

    Honey bees use a complex form of spatial referential communication. Their waggle dance communicates to nestmates the direction, distance, and quality of a resource by encoding celestial cues, retinal optic flow, and relative food value into motion

    Caste Transition and Reversion in Harpegnathos saltator Ant Colonies
    Authors:  Comzit Opachaloemphan, Francisco Carmona-Aldana and Hua Yan, date: 08/20/2023, view: 228, Q&A: 0

    Living organisms possess the ability to respond to environmental cues and adapt their behaviors and physiologies for survival. Eusocial insects, such as ants, bees, wasps, and termites, have evolved advanced sociality: living together in colonies

    Multi-color Flow Cytometry Protocol to Characterize Myeloid Cells in Mouse Retina Research
    Authors:  Wei Xiao, Rami A. Shahror, Carol A. Morris, Ruth B. Caldwell and Abdelrahman Y. Fouda, date: 08/20/2023, view: 457, Q&A: 0

    Myeloid cells, specifically microglia and macrophages, are activated in retinal diseases and can improve or worsen retinopathy outcomes based on their inflammatory phenotype. However, assessing the myeloid cell response after retinal injury in mice

    Detection and Quantification of Calcium Ions in the Endoplasmic Reticulum and Cytoplasm of Cultured Cells Using Fluorescent Reporter Proteins and ImageJ Software
    Authors:  Shunsuke Saito and Kazutoshi Mori, date: 08/20/2023, view: 473, Q&A: 0

    This protocol describes a method for detecting and quantifying calcium ions in the endoplasmic reticulum (ER) and cytoplasm of cultured cells using fluorescent reporter proteins and ImageJ software. Genetically engineered fluorescent reporter

    Binging from Food to Alcohol: A Sequential Interaction Between Binging Behaviors in Male Wistar Rats

    The development of excessive alcohol (ethanol) and/or highly palatable food self-administration is an essential task to elucidate the neurobiological mechanisms that underlie these behaviors. Previous work has highlighted that ethanol


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