Reprogramming Cancer Cells to Antigen-presenting Cells

    Cancer cells evade the immune system by downregulating antigen presentation. Although immune checkpoint inhibitors (ICI) and adoptive T-cell therapies revolutionized cancer treatment, their efficacy relies on the intrinsic immunogenicity of tumor

    Medullary Thymic Epithelial Cell Antigen-presentation Assays
    Authors:  Alexia Borelli, Cloé Zamit and Magali Irla, date: 11/05/2023, view: 217, Q&A: 0

    Medullary thymic epithelial cells (mTEC) are bona fide antigen-presenting cells that play a crucial role in the induction of T-cell tolerance. By their unique ability to express a broad range of tissue-restricted self-antigens, mTEC control the

    Three-dimensional Co-culture Model for Live Imaging of Pancreatic Islets, Immune Cells, and Neurons in Agarose Gel
    Authors:  Elke M. Muntjewerff, Vijay S. Josyula and Gustaf Christoffersson, date: 10/20/2023, view: 671, Q&A: 0

    During the onset of autoimmune diabetes, nerve–immune cell interactions seem to play an important role; however, there are currently no models to follow and interfere with these interactions over time in vivo or in vitro. Two-dimensional in

    Human Dendritic Cell Subset Isolation by Magnetic Bead Sorting: A Protocol to Efficiently Obtain Pure Populations

    Dendritic cells have been investigated for cell-based immunotherapy for various applications. The low abundance of dendritic cells in blood hampers their clinical application, resulting in the use of monocyte-derived dendritic cells as an

    Epicutaneous Application of Mannan Induces Psoriasis-like Inflammation in an Inbred Mouse Strain
    Authors:  Huimei Wu and Kutty Selva Nandakumar, date: 10/20/2023, view: 264, Q&A: 0

    Mannan from yeast induces psoriasis-like inflammation in the skin of inbred mouse strains. Limitations of available models led us to develop a new psoriasis model with a rapid disease onset, severe disease course, short duration, and a simple and

    Isolation and Analysis of B-cell Progenitors from Bone Marrow by Flow Cytometry
    Authors:  Hongchang Zhao, Roger Sciammas and Jacqueline H. Barlow, date: 10/05/2023, view: 335, Q&A: 0

    B cells play a critical role in host defense, producing antibodies in response to microbial infection. An inability to produce an effective antibody response leaves affected individuals prone to serious infection; therefore, proper B-cell

    Differentiation of Bone Marrow Monocytes into Alveolar Macrophages-like Cells through Co-culture with Lung Epithelial Cells and Group 2 Innate Lymphoid Cells
    Authors:  Pauline Loos, Thomas Marichal, Bénédicte Machiels and Laurent Gillet, date: 09/20/2023, view: 427, Q&A: 0

    During life, the embryonic alveolar macrophage (AM) population undergoes successive waves of depletion and replenishment in response to infectious and inflammatory episodes. While resident AMs are traditionally described as from embryonic origin,

    Double Staining with Fluorescent Tracers to Determine Myeloid Cell Migration of Leishmania-infected Cells from Mouse Skin to Lymphatic Tissues by Flow Cytometry
    Authors:  Ashanti C. Uscanga-Palomeque, E. Yaneth Osorio and Peter C. Melby, date: 09/20/2023, view: 438, Q&A: 0

    Immune cell trafficking in steady-state conditions and inflammatory cell recruitment into injured tissues is crucial for the surveillance of the immune system and the maintenance of body homeostasis. Tracking the cell journey from the infection site

    Functional Phenotyping of Lung Mouse CD4+ T Cells Using Multiparametric Flow Cytometry Analysis
    Authors:  Céline M. Maquet, Laurent Gillet and Bénédicte D. Machiels, date: 09/20/2023, view: 538, Q&A: 0

    Gammaherpesviruses such as Epstein-Barr virus (EBV) are major modulators of the immune responses of their hosts. In the related study (PMID: 35857578), we investigated the role for Ly6Chi monocytes in shaping the function of effector CD4+ T cells in

    Determination of Antibody Activity by Platelet Aggregation
    Authors:  Halina H.L. Leung, Jose Perdomo, Zohra Ahmadi and Beng H. Chong, date: 09/05/2023, view: 326, Q&A: 0

    Platelets play an important role in hemostasis by forming clots and stopping bleeding. In immune thrombotic conditions, platelets and leukocytes are aberrantly activated by pathogenic antibodies resulting in platelet aggregates and NETosis, leading


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