Protocols shared by authors via RaP service
Journal
On-scope validation of spatially controlled DNA hybridization
Authors: Kenneth Hu and Matthew Krummel
Date: Aug 10, 2022，Views：524
Original protocol used in research article：
ZipSeq: barcoding for real-time mapping of single cell transcriptomes
nature methods, Jul 6, 2022; DOI: 10.1038/s41592-020-0880-2
Thin layer chromatography (TLC)
Authors: Hannah Tabakh and Joshua J Woodward
Date: Aug 9, 2022，Views：568
Original protocol used in research article：
4-Hydroxy-2-nonenal antimicrobial toxicity is neutralized by an intracellular pathogen
eLife, May 6, 2021; DOI: 10.7554/eLife.59295
Single nuclei isolation of cardiac samples and processing on the 10X Genomics platform
Authors: Daniel Reichart, Eric L. Lindberg, Jonathan G. Seidman, Norbert Hubner and Christine E. Seidman
Date: Aug 9, 2022，Views：553
Original protocol used in research article：
Pathogenic variants damage cell composition and single cell transcription in cardiomyopathies
Science, Aug 5, 2022; DOI: 10.1126/science.abo1984
p53 reporter luciferase assays
Authors: Jay Vadgama and Yong Wu
Date: Aug 8, 2022，Views：624
Original protocol used in research article：
PP2Cδ inhibits p300-mediated p53 acetylation via ATM/BRCA1 pathway to impede DNA damage response in breast cancer
Science Advances, Oct 16, 2019; DOI: 10.1126/sciadv.aaw8417
Three-chamber task
Authors: Stefano Musardo and Camilla Bellone
Date: Aug 8, 2022，Views：574
Original protocol used in research article：
Oxytocin neurons mediate the effect of social isolation via the VTA circuits
eLife, Apr 22, 2022; DOI: 10.7554/eLife.73421
The luciferase reporter Sec UGA read through assay
Authors: Benjamin Yee Killam, Miljan Simonović and Paul R Copeland
Date: Aug 8, 2022，Views：560
Original protocol used in research article：
Structure of the mammalian ribosome as it decodes the selenocysteine UGA codon
Science, Jun 16, 2022; DOI: 10.1126/science.abg3875
Neonatal odor conditioning
Author: Hitoshi Sakano
Date: Aug 6, 2022，Views：553
Original protocol used in research article：
The olfactory critical period is determined by activity-dependent Sema7A/PlxnC1 signaling within glomeruli
eLife, Mar 29, 2021; DOI: 10.7554/eLife.65078
PKG-dependent TSC2 phosphorylation
Author: David A Kass
Date: Aug 5, 2022，Views：556
Original protocol used in research article：
Single serine on TSC2 exerts biased control over mTORC1 activation mediated by ERK1/2 but not Akt
Life Science Alliance, Mar 14, 2022; DOI: 10.26508/lsa.202101169
Primary source gender and cell line confirmations
Authors: Eben G Estell and Clifford J Rosen
Date: Aug 5, 2022，Views：572
Original protocol used in research article：
Irisin directly stimulates osteoclastogenesis and bone resorption in vitro and in vivo
eLife, Aug 11, 2020; DOI: 10.7554/eLife.58172
ISH and IHC
Authors: Minh Q Nguyen, Nicholas JP Ryba and Steve Davidson
Date: Aug 5, 2022，Views：606
Original protocol used in research article：
Single-nucleus transcriptomic analysis of human dorsal root ganglion neurons
eLife, Nov 26, 2021; DOI: 10.7554/eLife.71752
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