Bio-101 - Improve Research Reproducibility

Yeast strains and growth conditions

Authors: Pil Jung Kang and Hay-Oak Park
Date: Jul 27, 2022，Views：542

Original protocol used in research article：

Up-regulation of the Cdc42 GTPase limits the replicative life span of budding yeast
Molecular Biology of the Cell, Mar 11, 2022; DOI: 10.1091/mbc.E21-04-0208

Cell cycle analysis with BrdU and propidium iodide

Authors: Hui Zhao, Bernard Thienpont and Diether Lambrechts
Date: Jul 26, 2022，Views：639

Original protocol used in research article：

Mismatch repair deficiency endows tumors with a unique mutation signature and sensitivity to DNA double-strand breaks
eLife, Aug 1, 2014; DOI: 10.7554/eLife.02725

Quantitative Real-Time PCR

Authors: Xingjiang Yu and Dongsheng Guo
Date: Jul 25, 2022，Views：552

Original protocol used in research article：

Tenascin-C can Serve as an Indicator for the Immunosuppressive Microenvironment of Diffuse Low-Grade Gliomas
Frontiers in Immunology, Mar 16, 2022; DOI: 10.3389/fimmu.2022.824586

ATPase activity assays of SERCA reconstitutions

Authors: M'Lynn E Fisher and Howard S Young
Date: Jul 25, 2022，Views：662

Original protocol used in research article：

Dwarf open reading frame (DWORF) is a direct activator of the sarcoplasmic reticulum calcium pump SERCA
eLife, Jun 2, 2021; DOI: 10.7554/eLife.65545

Immunohistochemistry

Author: Sacha B Nelson
Date: Jul 25, 2022，Views：653

Original protocol used in research article：

Cortical RORβ is required for layer 4 transcriptional identity and barrel integrity
eLife, Aug 27, 2020; DOI: 10.7554/eLife.52370

4.5. Quantification of NETs by Fluorescence Spectrophotometry

Authors: Iwona Cichon, Weronika Ortmann and Elzbieta Kolaczkowska
Date: Jul 25, 2022，Views：550

Original protocol used in research article：

Metabolic Pathways Involved in Formation of Spontaneous and Lipopolysaccharide-Induced Neutrophil Extracellular Traps (NETs) Differ in Obesity and Systemic Inflammation
International Journal of Molecular Sciences, Jul 19, 2021; DOI: 10.3390/ijms22147718

Antisense oligonucleotide and LNA GapmeR transfections

Authors: Caroline Jane Ross and Igor Ulitsky
Date: Jul 24, 2022，Views：619

Original protocol used in research article：

Uncovering deeply conserved motif combinations in rapidly evolving noncoding sequences
Genome Biology, Jan 11, 2021; DOI: 10.1186/s13059-020-02247-1

Cell state transition inference

Authors: Liangtao Zheng, Shishang Qin, Xueda Hu and Zemin Zhang
Date: Jul 23, 2022，Views：640

Original protocol used in research article：

Pan-cancer single-cell landscape of tumor-infiltrating T cells
Science, Dec 17, 2021; DOI: 10.1126/science.abe6474

Flow Cytometry: Innate lymphoid cells in lung tissue

Author: Dominique M. A. Bullens
Date: Jul 23, 2022，Views：540

Original protocol used in research article：

Innate Lymphoid Cells Are Required to Induce Airway Hyperreactivity in a Murine Neutrophilic Asthma Model
Frontiers in Immunology, Mar 15, 2022; DOI: 10.3389/fimmu.2022.849155

Isolation of human macrophages and monocytes

Authors: Pizga Kumwenda and Rebecca A. Hall
Date: Jul 22, 2022，Views：589

Original protocol used in research article：

Estrogen promotes innate immune evasion of Candida albicans through inactivation of the alternative complement system
Cell Rep, Jan 4, 2022; DOI: 10.1016/j.celrep.2021.110183

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