Cell Biology


    Quantifying Single and Dual Channel Live Imaging Data: Kymograph Analysis of Organelle Motility in Neurons
    Authors:  Laura Digilio, Lloyd P. McMahon, Alois Duston, Chan Choo Yap and Bettina Winckler, date: 05/20/2023, view: 299, Q&A: 0

    Live imaging is commonly used to study dynamic processes in cells. Many labs carrying out live imaging in neurons use kymographs as a tool. Kymographs display time-dependent microscope data (time-lapsed images) in two-dimensional representations

    THRIFTY—A High-throughput Single Muscle Fiber Typing Method Based on Immunofluorescence Detection
    Authors:  Sebastian Edman, Oscar Horwath and William Apró, date: 05/20/2023, view: 226, Q&A: 0

    Skeletal muscle consists of a mixture of fiber types with different functional and metabolic characteristics. The relative composition of these muscle fiber types has implications for muscle performance, whole-body metabolism, and health. However,

    In vivo Drug Screening to Identify Anti-metastatic Drugs in Twist1a-ERT2 Transgenic Zebrafish
    Authors:  Joji Nakayama, Hideki Makinoshima and Zhiyuan Gong, date: 05/20/2023, view: 202, Q&A: 0

    Here, we present an in vivo drug screening protocol using a zebrafish model of metastasis for the identification of anti-metastatic drugs. A tamoxifen-controllable Twist1a-ERT2 transgenic zebrafish line was established to serve as a

    Implementation of a Drug Screening Platform to Target Gch1 Expression in Injured Mouse Dorsal Root Ganglion Neurons

    Management of neuropathic pain is notoriously difficult; current analgesics, including anti-inflammatory- and opioid-based medications, are generally ineffective and can pose serious side effects. There is a need to uncover non-addictive and safe

    Simultaneous Microendoscopic Calcium Imaging and EEG Recording of Mouse Brain during Sleep
    Authors:  Sasa Teng and Yueqing Peng, date: 05/05/2023, view: 160, Q&A: 0

    Sleep is a conserved biological process in the animal kingdom. Understanding the neural mechanisms underlying sleep state transitions is a fundamental goal of neurobiology, important for the development of new treatments for insomnia and other

    E15.5 Mouse Embryo Micro-CT Using a Bruker Skyscan 1172 Micro-CT
    Authors:  Elena Astanina, Sara Petrillo, Tullio Genova, Federico Mussano and Federico Bussolino, date: 05/05/2023, view: 161, Q&A: 0

    X-ray computed microtomography (µCT) is a powerful tool to reveal the 3D structure of tissues and organs. Compared with the traditional sectioning, staining, and microscopy image acquisition, it allows a better understanding of the morphology

    Modified Pseudo-Schiff Propidium Iodide for Staining the Shoot Apical Meristem in Arabidopsis
    Authors:  Ruiqi Li, Qi Li and Ligeng Ma, date: 05/05/2023, view: 279, Q&A: 0

    Visualization of cell structure with fluorescent dye for characterizing cell size, shape, and arrangement is a common method to study tissue morphology and morphogenesis. In order to observe shoot apical meristem (SAM) in Arabidopsis thaliana by

    Assessment of Chemosensory Response to Volatile Compounds in Healthy, Aged, and Neurodegenerative Caenorhabditis elegans Models
    Authors:  Cira Crespo and Roberto Grau, date: 05/05/2023, view: 165, Q&A: 0

    A basic function of the nervous system is to confer the ability to detect external stimuli and generate appropriate behavioral and physiological responses. These can be modulated when parallel streams of information are provided to the nervous

    Protocol for 3D Bioprinting Mesenchymal Stem Cell–derived Neural Tissues Using a Fibrin-based Bioink

    Three-dimensional bioprinting utilizes additive manufacturing processes that combine cells and a bioink to create living tissue models that mimic tissues found in vivo. Stem cells can regenerate and differentiate into specialized cell types, making

    Long-term in toto Imaging of Cellular Behavior during Nerve Injury and Regeneration
    Authors:  Weili Tian, Andrés González-Suarez and Hernán López-Schier, date: 05/05/2023, view: 261, Q&A: 0

    Accidental wounding of the peripheral nervous system leads to acute neural dysfunction. Normally, chronic deficits are overcome because peripheral nerves naturally regenerate. However, various genetic and metabolic defects can impair their natural


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