Cell Biology

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    A Robust Nanoparticle-based Magnetic Separation Method for Intact Lysosomes
    Authors:  The Son Le, Mari Takahashi and Shinya Maenosono, date: 07/05/2022, view: 35, Q&A: 0

    Lysosome isolation is a preresiquite for identifying lysosomal protein composition by mass spectroscopic analysis, to reveal lysosome functions, and their involvement in some diseases. Magnetic nanoparticle-based fractionation has received great

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    A β-glucuronidase (GUS) Based Bacterial Competition Assay to Assess Fine Differences in Fitness during Plant Infection
    Authors:  Julien S. Luneau, Laurent D. Noël, Emmanuelle Lauber and Alice Boulanger, date: 07/05/2022, view: 24, Q&A: 0

    Competition assays are a simple phenotyping strategy that confront two bacterial strains to evaluate their relative fitness. Because they are more accurate than single-strain growth assays, competition assays can be used to highlight slight

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    Whole-mount Senescence-Associated Beta-Galactosidase (SA-β-GAL) Activity Detection Protocol for Adult Zebrafish
    Authors:  Marta Marzullo, Mounir El Maï and Miguel Godinho Ferreira, date: 07/05/2022, view: 16, Q&A: 0

    Senescence-associated beta-galactosidase (SA-β-GAL) is an enzyme that accumulates in the lysosomes of senescent cells, where it hydrolyses β-galactosides. With p16, it represents a well-recognized biomarker used to assess senescence both

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    In vivo Characterization of Endogenous Protein Interactomes in Drosophila Larval Brain, Using a CRISPR/Cas9-based Strategy and BioID-based Proximity Labeling
    Authors:  Ezgi Uçkun, Georg Wolfstetter, Johannes Fuchs and Ruth H. Palmer, date: 07/05/2022, view: 22, Q&A: 0

    Understanding protein-protein interactions (PPIs) and interactome networks is essential to reveal molecular mechanisms mediating various cellular processes. The most common method to study PPIs in vivo is affinity purification combined with mass

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    Automated Quantification of Multiple Cell Types in Fluorescently Labeled Whole Mouse Brain Sections Using QuPath

    The quantification of labeled cells in tissue sections is crucial to the advancement of biological knowledge. Traditionally, this was a tedious process, requiring hours of careful manual counting in small portions of a larger tissue section. To

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    Experimental Models for Cold Exposure of Muscle in vitro and in vivo

    Work in cold environments may have a significant impact on occupational health. In these and similar situations, cold exposure localized to the extremities may reduce the temperature of underlying tissues. To investigate the molecular effects of

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    Quantitative Live Confocal Imaging in Aquilegia Floral Meristems
    Authors:  Ya Min, Stephanie J. Conway and Elena M. Kramer, date: 06/20/2022, view: 992, Q&A: 0

    In this study, we present a detailed protocol for live imaging and quantitative analysis of floral meristem development in Aquilegia coerulea, a member of the buttercup family (Ranunculaceae). Using confocal microscopy and the image analysis

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    Fluorescent Labeling of Small Extracellular Vesicles (EVs) Isolated from Conditioned Media
    Authors:  John Santelices, Mark Ou, Winnie W. Hui, Gustavo H. B. Maegawa and Mariola J. Edelmann, date: 06/20/2022, view: 1573, Q&A: 0

    Extracellular vesicles (EVs), such as exosomes, are produced by all known eukaryotic cells, and constitute essential means of intercellular communication. Recent studies have unraveled the important roles of EVs in migrating to specific sites and

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    Labelling of Active Transcription Sites with Argonaute NRDE-3—Image Active Transcription Sites in vivo in Caenorhabditis elegans
    Authors:  Antoine Barrière and Vincent Bertrand, date: 06/05/2022, view: 519, Q&A: 0

    Live labelling of active transcription sites is critical to our understanding of transcriptional dynamics. In the most widely used method, RNA sequence MS2 repeats are added to the transcript of interest, on which fluorescently tagged Major Coat

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    Electroporation of Small Interfering RNAs into Tibialis Anterior Muscles of Mice
    Authors:  Anna Stephan, Flavia A. Graca, Liam C. Hunt and Fabio Demontis, date: 06/05/2022, view: 787, Q&A: 0

    Aging and wasting of skeletal muscle reduce organismal fitness. Regrettably, only limited interventions are currently available to address this unmet medical need. Many methods have been developed to study this condition, including the

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