Protocols shared by authors via RaP service
Journal
Immunostaining
Authors: Amanda C Ignacz and Clarissa A Henry
Date: Aug 29, 2022，Views：580
Original protocol used in research article：
Beneficial impacts of neuromuscular electrical stimulation on muscle structure and function in the zebrafish model of Duchenne muscular dystrophy
eLife, ; DOI: 10.7554/eLife.62760
Luciferase assay
Authors: Jochen Winterer and Gerhard Schratt
Date: Aug 29, 2022，Views：715
Original protocol used in research article：
MicroRNA-138 controls hippocampal interneuron function and short-term memory in mice
eLife, Mar 15, 2022; DOI: 10.7554/eLife.74056
Genotyping dHT mice and real-time qPCR
Authors: Francesco Zorzato and Alexis Ruiz
Date: Aug 29, 2022，Views：584
Original protocol used in research article：
Improvement of muscle strength in a mouse model for congenital myopathy treated with HDAC and DNA methyltransferase inhibitors
eLife, Mar 3, 2022; DOI: 10.7554/eLife.73718
2.3.1. The Cognitive Impulsivity Suite (CIS)
Authors: Katharina Voigt and Antonio Verdejo-Garcia
Date: Aug 28, 2022，Views：636
Original protocol used in research article：
The Hunger Games: Homeostatic State-Dependent Fluctuations in Disinhibition Measured with a Novel Gamified Test Battery
Nutrients, Jun 10, 2021; DOI: 10.3390/nu13062001
Flow cytometry
Authors: Shuang Wang, Wei Wei and Guanghui Ma
Date: Aug 26, 2022，Views：544
Original protocol used in research article：
Macrophage-tumor chimeric exosomes accumulate in lymph node and tumor to activate the immune response and the tumor microenvironment
Science Translational Medicine, Oct 13, 2021; DOI: 10.1126/scitranslmed.abb6981
In vivo and ex vivo imaging
Authors: Shuang Wang, Wei Wei and Guanghui Ma
Date: Aug 26, 2022，Views：536
Original protocol used in research article：
Macrophage-tumor chimeric exosomes accumulate in lymph node and tumor to activate the immune response and the tumor microenvironment
Science Translational Medicine, Oct 13, 2021; DOI: 10.1126/scitranslmed.abb6981
T cell receptor beta chain (TRB) sequencing of CD8 T cells from LNs
Authors: Shuang Wang, Wei Wei and Guanghui Ma
Date: Aug 26, 2022，Views：539
Original protocol used in research article：
Macrophage-tumor chimeric exosomes accumulate in lymph node and tumor to activate the immune response and the tumor microenvironment
Science Translational Medicine, Oct 13, 2021; DOI: 10.1126/scitranslmed.abb6981
Immunofluorescence (Tryptase and PGP9.5) and immunohistochemistry (H4R)
Authors: Giada De Palma, Chiko Shimbori and Premysl Bercik
Date: Aug 24, 2022，Views：547
Original protocol used in research article：
Histamine production by the gut microbiota induces visceral hyperalgesia through histamine 4 receptor signaling in mice
Science Translational Medicine, Jul 27, 2022; DOI: 10.1126/scitranslmed.abj1895
Production of anti–CTLA-4×SIRPα heterodimer
Authors: Anli Zhang and Yang-Xin Fu
Date: Aug 24, 2022，Views：593
Original protocol used in research article：
Dual targeting of CTLA-4 and CD47 on Treg cells promotes immunity against solid tumors
Science Translational Medicine, Aug 4, 2021; DOI: 10.1126/scitranslmed.abg8693
Preparation of nano-Ag@erythrosomes
Authors: Zhuang Liu, Zhen Gu and Chao Wang
Date: Aug 22, 2022，Views：551
Original protocol used in research article：
Red blood cell–derived nanoerythrosome for antigen delivery with enhanced cancer immunotherapy
Science Advances, Oct 23, 2019; DOI: 10.1126/sciadv.aaw6870
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