Protocols shared by authors via RaP service
Journal
Production of Nanobody 35
Authors: Fenghui Zhao, H Eric Xu, Dehua Yang, Lihua Zhao and Ming-Wei Wang
Date: Dec 21, 2025，Views：2
Original protocol used in research article：
Structural insights into hormone recognition by the human glucose-dependent insulinotropic polypeptide receptor
eLife, Jul 13, 2021; DOI: 10.7554/eLife.68719
Co-immunoprecipitation and western blotting
Author: Yukiko M Yamashita
Date: Dec 18, 2025，Views：0
Original protocol used in research article：
Alstrom syndrome gene is a stem-cell-specific regulator of centriole duplication in the Drosophila testis
eLife, Sep 23, 2020; DOI: 10.7554/eLife.59368
Flow cytometric analysis
Authors: Ambre Riba and Mathias W. Hornef
Date: Dec 17, 2025，Views：4
Original protocol used in research article：
Disturbed gut microbiota and bile homeostasis in Giardia-infected mice contributes to metabolic dysregulation and growth impairment
Science Translational Medicine, Oct 13, 2020; DOI: 10.1126/scitranslmed.aay7019
Direct co-culture of parasites
Author: Natalia Hernan de Miguel
Date: Dec 11, 2025，Views：40
Original protocol used in research article：
Role of cytoneme structures and extracellular vesicles in Trichomonas vaginalis parasite-parasite communication
eLife, May 2, 2023; DOI: 10.7554/eLife.86067
Nanobody screening by phage display
Authors: baolong xia and Norbert Perrimon
Date: Dec 3, 2025，Views：465
Original protocol used in research article：
Phage-displayed synthetic library and screening platform for nanobody discovery
eLife, Aug 1, 2025; DOI: 10.7554/eLife.105887
Synthesis of thanatin and derivatives 1 to 7
Authors: Matthias Schuster, Daniel Obrecht, Mohammed Benghezal, Oliver Zerbe, Gregory Ubert, Emile Brabet and Anatol Luther
Date: Dec 2, 2025，Views：395
Original protocol used in research article：
Peptidomimetic antibiotics disrupt the lipopolysaccharide transport bridge of drug-resistant Enterobacteriaceae
Science Advances, May 24, 2023; DOI: 10.1126/sciadv.adg3683
Maintenance of Drosophila S2 cells and CO2 exposure
Author: Masahiko Shigemura
Date: Nov 24, 2025，Views：587
Original protocol used in research article：
Elevated CO2 regulates the Wnt signaling pathway in mammals, Drosophila melanogaster and Caenorhabditis elegans
Scientific Reports, Dec 3, 2019; DOI: 10.1038/s41598-019-54683-0
In vitro culture of adult mouse DRG neurons
Authors: Pavel Hanč and Ulrich H. von Andrian
Date: Nov 24, 2025，Views：511
Original protocol used in research article：
Multimodal control of dendritic cell functions by nociceptors
Science, Mar 31, 2023; DOI: 10.1126/science.abm5658
Cryopreservation
Author: Jean-Baptiste Raina
Date: Nov 13, 2025，Views：556
Original protocol used in research article：
Subcellular tracking reveals the location of dimethylsulfoniopropionate in microalgae and visualises its uptake by marine bacteria
eLife, Apr 4, 2017; DOI: 10.7554/eLife.23008
Modeling and computational docking
Authors: Eva Susanne Istvan, Daniel E Goldberg and Elizabeth A Winzeler
Date: Nov 12, 2025，Views：535
Original protocol used in research article：
Cytoplasmic isoleucyl tRNA synthetase as an attractive multistage antimalarial drug target
Science Translational Medicine, Mar 8, 2023; DOI: 10.1126/scitranslmed.adc9249
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