Protocols shared by authors via RaP service
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Purification of endogenous lysyl oxidase (LOX) from MEF cell culture
Author: Tomoyuki Nakamura
Date: Nov 28, 2024，Views：73
Original protocol used in research article：
A matricellular protein fibulin-4 is essential for the activation of lysyl oxidase
Science Advances, Nov 24, 2020; DOI: 10.1126/sciadv.abc1404
Taq I Chromosome Conformation Capture (Taq I-3C)
Authors: Linda S Rubio and David S Gross
Date: Nov 20, 2024，Views：102
Original protocol used in research article：
Heat Shock Factor 1 forms nuclear condensates and restructures the yeast genome before activating target genes
eLife, Oct 15, 2024; DOI: 10.7554/eLife.92464
iCVD synthesis protocol
Authors: Pengyu Chen and Rong Yang
Date: Nov 10, 2024，Views：57
Original protocol used in research article：
An imidazolium-based zwitterionic polymer for antiviral and antibacterial dual functional coatings
Science Advances, Jan 14, 2022; DOI: 10.1126/sciadv.abl8812
Drosophila ovary extraction, fixation and immunostaining protocol
Authors: Wen Lu and Vladimir I. Gelfand
Date: Oct 31, 2024，Views：108
Original protocol used in research article：
A novel mechanism of bulk cytoplasmic transport by cortical dynein in Drosophila ovary
eLife, Feb 16, 2022; DOI: 10.7554/eLife.75538
4.4. Eriochrome Cyanine Staining
Authors: Rocío Benítez-Fernández, Ana Martínez and Fernando de Castro
Date: Oct 28, 2024，Views：85
Original protocol used in research article：
Dynamics of Central Remyelination and Treatment Evolution in a Model of Multiple Sclerosis with Optic Coherence Tomography
International Journal of Molecular Sciences, Feb 28, 2021; DOI: 10.3390/ijms22052440
Proteoliposome-based in vitro transport assays
Authors: Shamayeeta Ray and Rachelle Gaudet
Date: Oct 27, 2024，Views：177
Original protocol used in research article：
High-resolution structures with bound Mn2+ and Cd2+ map the metal import pathway in an Nramp transporter
eLife, Apr 11, 2023; DOI: 10.7554/eLife.84006
Co-immunoprecipitation from S2 cells
Author: Richard G Fehon
Date: Oct 26, 2024，Views：70
Original protocol used in research article：
Negative feedback couples Hippo pathway activation with Kibra degradation independent of Yorkie-mediated transcription
eLife, Feb 8, 2021; DOI: 10.7554/eLife.62326
Quantification of cytosolic DNA
Authors: Haitao Wen, JIANWEN CHEN and Hong Dong
Date: Oct 24, 2024，Views：95
Original protocol used in research article：
Inhibition of O-GlcNAc transferase activates type I interferon-dependent antitumor immunity by bridging cGAS-STING pathway
eLife, Oct 4, 2024; DOI: 10.7554/eLife.94849
Cell culture
Author: Yoko Nakai-Futatsugi
Date: Oct 12, 2024，Views：47
Original protocol used in research article：
Pigmentation level of human iPSC-derived RPE does not indicate a specific gene expression profile
eLife, May 9, 2024; DOI: 10.7554/eLife.92510
Expansion of hPS Cell Derived Pancreatic Progenitors
Author: Anthony Gavalas
Date: Oct 9, 2024，Views：129
Original protocol used in research article：
Regulation of multiple signaling pathways promotes the consistent expansion of human pancreatic progenitors in defined conditions
eLife, Jan 5, 2024; DOI: 10.7554/eLife.89962
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