Protocols shared by authors via RaP service
Journal
Plant growth Conditions
Authors: Kenneth D. Birnbaum and Carlos Ortiz-Ramírez
Date: Apr 24, 2025，Views：362
Original protocol used in research article：
Ground tissue circuitry regulates organ complexity in maize and Setaria
Science, Dec 2, 2021; DOI: 10.1126/science.abj2327
Source code
Author: Priscila S Rothier
Date: Apr 16, 2025，Views：34
Original protocol used in research article：
Mammalian forelimb evolution is driven by uneven proximal-to-distal morphological diversity
eLife, Jan 26, 2023; DOI: 10.7554/eLife.81492
PBMC flow cytometry
Authors: Eula Graciele Amorim Neves, Walderez Ornelas Dutra and Maria Bellio
Date: Mar 28, 2025，Views：502
Original protocol used in research article：
Cytotoxic CD4+ T cells driven by T-cell intrinsic IL-18R/MyD88 signaling predominantly infiltrate Trypanosoma cruzi-infected hearts
eLife, ; DOI: 10.7554/eLife.74636
Alizarin red staining and imaging of zebrafish larvae and adults
Authors: Sophie Debaenst and Andy Willaert
Date: Mar 25, 2025，Views：396
Original protocol used in research article：
Crispant analysis in zebrafish as a tool for rapid functional screening of disease-causing genes for bone fragility
eLife, Jan 16, 2025; DOI: 10.7554/eLife.100060
Conformational stability assay and immunoblotting
Authors: Selene Seoyun Lee, Livia Civitelli and Laura Parkkinen
Date: Mar 20, 2025，Views：314
Original protocol used in research article：
Brain-derived and in vitro-seeded alpha-synuclein fibrils exhibit distinct biophysical profiles
eLife, Nov 25, 2024; DOI: 10.7554/eLife.92775
Antibodies
Authors: Marcus Buggert, André Perez-Potti and Takuya Sekine
Date: Mar 14, 2025，Views：310
Original protocol used in research article：
TOX is expressed by exhausted and polyfunctional human effector memory CD8+ T cells
Science Immunology, Jul 2, 2020; DOI: 10.1126/sciimmunol.aba7918
Neural differentiation assay
Authors: Zhiguo Zhang and Xiaowei Xu
Date: Mar 9, 2025，Views：418
Original protocol used in research article：
Mcm2 promotes stem cell differentiation via its ability to bind H3-H4
eLife, Nov 10, 2022; DOI: 10.7554/eLife.80917
Single-cell RNA-seq
Authors: Kenneth D. Birnbaum and Carlos Ortiz-Ramírez
Date: Feb 27, 2025，Views：39
Original protocol used in research article：
Ground tissue circuitry regulates organ complexity in maize and Setaria
Science, Dec 2, 2021; DOI: 10.1126/science.abj2327
Kim-1 targeting ability
Authors: Xuefang Lou, Saiping Jiang and Yongzhong Du
Date: Feb 26, 2025，Views：364
Original protocol used in research article：
ROS-responsive chitosan-SS31 prodrug for AKI therapy via rapid distribution in the kidney and long-term retention in the renal tubule
Science Advances, Oct 8, 2020; DOI: 10.1126/sciadv.abb7422
PRPS activity assay
Authors: Huan-Huan Hu, Guang-Ming Lu and Ji-Long Liu
Date: Feb 20, 2025，Views：436
Original protocol used in research article：
Filamentation modulates allosteric regulation of PRPS
eLife, ; DOI: 10.7554/eLife.79552
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