Protocols shared by authors via RaP service
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Identification of the bcd1-2 allele
Author: Jacek Gaertig
Date: Jan 5, 2025，Views：296
Original protocol used in research article：
The Tetrahymena bcd1 mutant implicates endosome trafficking in ciliate, cortical pattern formation
Molecular Biology of the Cell, Jun 20, 2023; DOI: 10.1091/mbc.E22-11-0501
Liver Cholesterol and Triglycerides Measurements
Authors: Shunxing Rong and Jay D. Horton
Date: Dec 31, 2024，Views：295
Original protocol used in research article：
Expression of SREBP-1c Requires SREBP-2-mediated Generation of a Sterol Ligand for LXR in Livers of Mice
eLife, Feb 28, 2017; DOI: 10.7554/eLife.25015
Hemoglobin detection by HPLC
Authors: Nithin Sam Ravi and Kumarasamypet M Mohankumar
Date: Dec 30, 2024，Views：306
Original protocol used in research article：
Identification of novel HPFH-like mutations by CRISPR base editing that elevate the expression of fetal hemoglobin
eLife, Feb 11, 2022; DOI: 10.7554/eLife.65421
Lysosome immunoprecipitation in C. elegans
Authors: Yong Yu and Meng C Wang
Date: Dec 20, 2024，Views：241
Original protocol used in research article：
Organelle proteomic profiling reveals lysosomal heterogeneity in association with longevity
eLife, Jan 19, 2024; DOI: 10.7554/eLife.85214
Serum Bactericidal Assay
Authors: Jutamas Shaughnessy and Sanjay Ram
Date: Dec 18, 2024，Views：18
Original protocol used in research article：
Development of Complement Factor H–Based Immunotherapeutic Molecules in Tobacco Plants Against Multidrug-Resistant Neisseria gonorrhoeae
Frontiers in Immunology, Oct 26, 2020; DOI: 10.3389/fimmu.2020.583305
Worm culture and imaging
Authors: Suhong Xu and Weirui Ma
Date: Dec 18, 2024，Views：202
Original protocol used in research article：
Enhanced single RNA imaging reveals dynamic gene expression in live animals
eLife, Mar 3, 2023; DOI: 10.7554/eLife.82178
LFn fusion protein preparation
Author: Jiahuai Han
Date: Dec 16, 2024，Views：122
Original protocol used in research article：
NLRC4 inflammasome–dependent cell death occurs by a complementary series of three death pathways and determines lethality in mice
Science Advances, Oct 22, 2021; DOI: 10.1126/sciadv.abi9471
Image alignment
Authors: John Darby Cole and Sebastian Jessberger
Date: Dec 6, 2024，Views：96
Original protocol used in research article：
Characterization of the neurogenic niche in the aging dentate gyrus using iterative immunofluorescence imaging
eLife, Jan 28, 2022; DOI: 10.7554/eLife.68000
Protein expression and purification
Authors: Anthony Maxwell and Keir C Neuman
Date: Dec 5, 2024，Views：127
Original protocol used in research article：
Topoisomerase VI is a chirally-selective, preferential DNA decatenase
eLife, Jan 25, 2022; DOI: 10.7554/eLife.67021
Chromatin immunoprecipitation_by_Claudio Cantù
Author: Claudio Cantù
Date: Nov 29, 2024，Views：85
Original protocol used in research article：
TBX3 acts as tissue-specific component of the Wnt/β-catenin transcriptional complex
eLife, Aug 18, 2020; DOI: 10.7554/eLife.58123
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