Protocols shared by authors via RaP service
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CitH3‐DNA ELISA
Authors: Yuanlin Song, Qiaoliang Zhu and Jian Zhou
Date: Nov 2, 2025，Views：23
Original protocol used in research article：
Lianhua Qingke ameliorates lipopolysaccharide‐induced lung injury by inhibiting neutrophil extracellular traps formation and pyroptosis
Pulmonary Circulation, Oct 6, 2023; DOI: 10.1002/pul2.12295
Genotyping CCR7-flox mice
Author: Milena Bogunovic
Date: Oct 27, 2025，Views：10
Original protocol used in research article：
Gut-resident CX3CR1hi macrophages induce tertiary lymphoid structures and IgA response in situ
Science Immunology, Apr 9, 2020; DOI: 10.1126/sciimmunol.aax0062
Organoid Seeding Protocol on Chips for MEA recordings
Authors: Michele Bertacchi and Michèle Studer
Date: Oct 26, 2025，Views：397
Original protocol used in research article：
FGF8-mediated gene regulation affects regional identity in human cerebral organoids
eLife, Nov 1, 2024; DOI: 10.7554/eLife.98096
Sample preparation for light sheet microscopy
Authors: Johannes Krug, Christoph Englert, Birgit Perner, Carolin Albertz, Hanna Mörl Mörl and Vera Hopfenmüller
Date: Oct 17, 2025，Views：216
Original protocol used in research article：
Generation of a transparent killifish line through multiplex CRISPR/Cas9mediated gene inactivation
eLife, Feb 23, 2023; DOI: 10.7554/eLife.81549
In vivo light sheet imaging
Authors: Johannes Krug, Christoph Englert, Birgit Perner, Carolin Albertz, Hanna Mörl Mörl and Vera Hopfenmüller
Date: Oct 17, 2025，Views：23
Original protocol used in research article：
Generation of a transparent killifish line through multiplex CRISPR/Cas9mediated gene inactivation
eLife, Feb 23, 2023; DOI: 10.7554/eLife.81549
Fluorescence-based ion transport assays using Proteoliposomes
Authors: Cristina Manatschal and Karthik Ramanadane
Date: Sep 20, 2025，Views：282
Original protocol used in research article：
Structural and functional properties of a magnesium transporter of the SLC11/NRAMP family
eLife, Jan 10, 2022; DOI: 10.7554/eLife.74589
Mounting
Authors: John Darby Cole and Sebastian Jessberger
Date: Sep 18, 2025，Views：162
Original protocol used in research article：
Characterization of the neurogenic niche in the aging dentate gyrus using iterative immunofluorescence imaging
eLife, Jan 28, 2022; DOI: 10.7554/eLife.68000
Tracer injections
Authors: Natasha O'Brown, Sean G Megason and Chenghua Gu
Date: Sep 12, 2025，Views：173
Original protocol used in research article：
Suppression of transcytosis regulates zebrafish blood-brain barrier function
eLife, Aug 20, 2019; DOI: 10.7554/eLife.47326
Principle component analysis (PCA)
Authors: Kingsley Wu, Ta I Hung and Chia-en A Chang
Date: Sep 11, 2025，Views：181
Original protocol used in research article：
PROTAC-induced protein structural dynamics in targeted protein degradation
eLife, Feb 27, 2025; DOI: 10.7554/eLife.101127
ChIP-seq analysis
Authors: Liang Xu, Ye Chen and Tuan Zea Tan
Date: Sep 9, 2025，Views：136
Original protocol used in research article：
Topography of transcriptionally active chromatin in glioblastoma
Science Advances, Apr 30, 2021; DOI: 10.1126/sciadv.abd4676
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