Protocols shared by authors via RaP service
Journal
Myeloid progenitor cell differentiation and microglial cell maturation
Authors: Wenxin Ma, Wai T Wong and Wei Li
Date: Feb 18, 2025，Views：379
Original protocol used in research article：
Human-induced pluripotent stem cell-derived microglia integrate into mouse retina and recapitulate features of endogenous microglia
eLife, Nov 8, 2024; DOI: 10.7554/eLife.90695
DNA Fluorescence in situ hybridisation (FISH)
Authors: Karin Purshouse and Wendy A Bickmore
Date: Feb 17, 2025，Views：448
Original protocol used in research article：
Oncogene expression from extrachromosomal DNA is driven by copy number amplification and does not require spatial clustering in glioblastoma stem cells
eLife, Dec 7, 2022; DOI: 10.7554/eLife.80207
Acid urea polyacrylamide gel electrophoresis (AUPAGE) and Northern analysis
Authors: Sunita Maharjan, Isao Masuda and Ya-Ming Hou
Date: Feb 4, 2025，Views：649
Original protocol used in research article：
Loss of N1-methylation of G37 in tRNA induces ribosome stalling and reprograms gene expression
eLife, Aug 12, 2021; DOI: 10.7554/eLife.70619
Measurement of the fluorescence intensity of TCSn:GFP in the Arabidopsis root epidermis
Authors: Naoki Takahashi and Masaaki Umeda
Date: Feb 2, 2025，Views：33
Original protocol used in research article：
Alterations in hormonal signals spatially coordinate distinct responses to DNA double-strand breaks in Arabidopsis roots
Science Advances, Jun 15, 2021; DOI: 10.1126/sciadv.abg0993
Molecular dynamics simulations
Authors: Anja Henning , Serdal Kirmizialtin and Dave Thirumalai
Date: Jan 30, 2025，Views：359
Original protocol used in research article：
Differences in ion-RNA binding modes due to charge density variations explain the stability of RNA in monovalent salts
Science Advances, Jul 20, 2022; DOI: 10.1126/sciadv.abo1190
Protein expression with unnatural amino acids in Pseudomonas aeruginosa
Authors: Eden Ozer and Lital Alfonta
Date: Jan 16, 2025，Views：364
Original protocol used in research article：
An inside look at a biofilm: Pseudomonas aeruginosa flagella biotracking
Science Advances, Jun 11, 2021; DOI: 10.1126/sciadv.abg8581
Isolation of sweat glands from mice
Authors: Makiko Kashio and Makoto Tominaga
Date: Jan 16, 2025，Views：343
Original protocol used in research article：
Involvement of TRPV4 in temperature-dependent perspiration in mice
eLife, Jul 4, 2024; DOI: 10.7554/eLife.92993
The preparation of lung organoid-derived monolayers
Authors: Courtney Tindle and Pradipta Ghosh
Date: Jan 13, 2025，Views：325
Original protocol used in research article：
Adult stem cell-derived complete lung organoid models emulate lung disease in COVID-19
eLife, Aug 13, 2021; DOI: 10.7554/eLife.66417
FRET assay
Authors: André Veillette, Bin Li and Zhenghai Tang
Date: Jan 10, 2025，Views：311
Original protocol used in research article：
Cis interactions between CD2 and its ligands on T cells are required for T cell activation
Science Immunology, Aug 5, 2022; DOI: 10.1126/sciimmunol.abn6373
Alexander staining of Arabidopsis anthers
Authors: Upendo Lupanga and Karin Schumacher
Date: Jan 7, 2025，Views：443
Original protocol used in research article：
The Arabidopsis V-ATPase is localized to the TGN/EE via a seed plant-specific motif
eLife, Nov 25, 2020; DOI: 10.7554/eLife.60568
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