Protocols shared by authors via RaP service
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Protocol for FilmTracer™ SYPRO® Ruby biofilm matrix stain
Author: Ronit Vogt Sionov
Date: Sep 23, 2024，Views：130
Original protocol used in research article：
Tooth mousse containing casein phosphopeptide-amorphous calcium phosphate prevents biofilm formation of Streptococcus mutans
BMC Oral Health, Mar 19, 2021; DOI: 10.1186/s12903-021-01502-6
Primary keratinocyte cell culture
Author: Cheryl L Stucky
Date: Sep 3, 2024，Views：139
Original protocol used in research article：
Keratinocytes mediate innocuous and noxious touch via ATP-P2X4 signaling
eLife, Jan 16, 2018; DOI: 10.7554/eLife.31684
Isolation of PDGFRβ+ REPs
Authors: Guangfeng Geng, Quan Chen, Yushan Zhu and Lihong Shi
Date: Aug 15, 2024，Views：101
Original protocol used in research article：
Receptor-mediated mitophagy regulates EPO production and protects against renal anemia
eLife, May 4, 2021; DOI: 10.7554/eLife.64480
In Vivo T Cell Migration Assay
Authors: Francisco Osorio-Barrios and Rodrigo Pacheco
Date: Aug 8, 2024，Views：70
Original protocol used in research article：
The Heteromeric Complex Formed by Dopamine Receptor D5 and CCR9 Leads the Gut Homing of CD4+ T Cells Upon Inflammation
Cellular and Molecular Gastroenterology and Hepatology, Apr 20, 2021; DOI: 10.1016/j.jcmgh.2021.04.006
Primers for validation of Rosa-Gpr56 mice
Authors: Diankun Yu and Xianhua Piao
Date: Aug 1, 2024，Views：76
Original protocol used in research article：
Microglial GPR56 is the molecular target of maternal immune activation-induced parvalbumin-positive interneuron deficits
Science Advances, May 6, 2022; DOI: 10.1126/sciadv.abm2545
Stable expression in HeLa cells and MEFs by retrovirus infection
Authors: Hayashi Yamamoto and Noboru Mizushima
Date: Jul 23, 2024，Views：136
Original protocol used in research article：
A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag
eLife, ; DOI: 10.7554/eLife.78923
Protease expression and purification
Authors: Celia A Schiffer, Ronald Swanstrom and Ean Spielvogel
Date: Jul 22, 2024，Views：80
Original protocol used in research article：
Selection of HIV-1 for resistance to fifth-generation protease inhibitors reveals two independent pathways to high-level resistance
eLife, Mar 15, 2023; DOI: 10.7554/eLife.80328
Determination of copper concentration in rice by ICP-OES
Authors: Yi Li and Shengze Yao
Date: Jul 21, 2024，Views：60
Original protocol used in research article：
The key micronutrient copper orchestrates broad-spectrum virus resistance in rice
Science Advances, Jul 1, 2022; DOI: 10.1126/sciadv.abm0660
Neuronal cultures
Authors: Guiping Wang, Marc Tessier-Lavigne and Xiaowei Zhuang
Date: Jul 17, 2024，Views：63
Original protocol used in research article：
Structural plasticity of actin-spectrin membrane skeleton and functional role of actin and spectrin in axon degeneration
eLife, May 1, 2019; DOI: 10.7554/eLife.38730
Prediction of functional impact of variants using PROVEAN
Authors: Stefano G Giulieri, Timothy P Stinear and Benjamin P Howden
Date: Jul 14, 2024，Views：57
Original protocol used in research article：
Niche-specific genome degradation and convergent evolution shaping Staphylococcus aureus adaptation during severe infections
eLife, ; DOI: 10.7554/eLife.77195
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