Protocols shared by authors via RaP service
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Fluorescent BODIPY-FL vancomycin staining
Authors: Moagi Tube Shaku and Bavesh D Kana
Date: Aug 8, 2025，Views：223
Original protocol used in research article：
A modified BCG with depletion of enzymes associated with peptidoglycan amidation induces enhanced protection against tuberculosis in mice
eLife, Apr 19, 2024; DOI: 10.7554/eLife.89157
Detection of CMV pp65-specific T cells by intracellular staining with anti-INF-γ-PE
Author: David Klatzmann
Date: Aug 1, 2025，Views：205
Original protocol used in research article：
Human thymopoiesis produces polyspecific CD8+ α/β T cells responding to multiple viral antigens
eLife, Mar 30, 2023; DOI: 10.7554/eLife.81274
EdU immunostaining to detect dividing cells
Author: Richard W Naylor
Date: Jul 29, 2025，Views：204
Original protocol used in research article：
A novel mechanism of gland formation in zebrafish involving transdifferentiation of renal epithelial cells and live cell extrusion
eLife, Nov 5, 2018; DOI: 10.7554/eLife.38911
Expression and purification protocol of ASK1 (residues 88-973, consisting of TBD, CRR, and KD)
Authors: Karolina Honzejkova, Veronika Obsilova and Tomas Obsil
Date: Jul 28, 2025，Views：204
Original protocol used in research article：
The cryo-EM structure of ASK1 reveals an asymmetric architecture allosterically modulated by TRX1
eLife, Mar 27, 2024; DOI: 10.7554/eLife.95199
Protocol for GST-Tagged Protein Purification
Authors: Donghai Wang, Hudan Liu, Kaiwei Liang and Guoliang Qing
Date: Jul 25, 2025，Views：217
Original protocol used in research article：
The super elongation complex drives transcriptional addiction in MYCN-amplified neuroblastoma
Science Advances, Mar 29, 2023; DOI: 10.1126/sciadv.adf0005
Total nucleated cells preparation enriching HSPC and S100A12high Neu2 for scRNA-seq
Authors: Yingying Huo, Linjie Wu, Erlie Jiang, Sha Hao and Tao Cheng
Date: Jul 14, 2025，Views：202
Original protocol used in research article：
Single-cell dissection of human hematopoietic reconstitution after allogeneic hematopoietic stem cell transplantation
Science Immunology, Mar 17, 2023; DOI: 10.1126/sciimmunol.abn6429
In vivo metal transport assays
Author: Rachelle Gaudet
Date: Jul 7, 2025，Views：278
Original protocol used in research article：
Structures in multiple conformations reveal distinct transition metal and proton pathways in an Nramp transporter
eLife, Feb 4, 2019; DOI: 10.7554/eLife.41124
TEER measurement
Authors: Lingjun Tong, Huaxi Yi and Jiong-Wei Wang
Date: Jun 20, 2025，Views：401
Original protocol used in research article：
Milk-derived extracellular vesicles protect intestinal barrier integrity in the gut-liver axis
Science Advances, Apr 12, 2023; DOI: 10.1126/sciadv.ade5041
MD simulations
Author: Pierre Cazade
Date: Jun 20, 2025，Views：32
Original protocol used in research article：
Modulating the pro-apoptotic activity of cytochrome c at a biomimetic electrified interface
Science Advances, Nov 5, 2021; DOI: 10.1126/sciadv.abg4119
Generation of CRISPR/Cas9-induced BMD mice
Authors: Daigo Miyazaki, Mitsuto Sato, Naoko Shiba, Takahiro Yoshizawa and Akinori Nakamura
Date: Jun 18, 2025，Views：369
Original protocol used in research article：
Becker muscular dystrophy mice showed site-specific decay of type IIa fibers with capillary change in skeletal muscle
eLife, Mar 17, 2025; DOI: 10.7554/eLife.100665
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