Bio-101 - Improve Research Reproducibility

In vivo metal transport assays

Author: Rachelle Gaudet
Date: Jul 7, 2025，Views：257

Original protocol used in research article：

Structures in multiple conformations reveal distinct transition metal and proton pathways in an Nramp transporter
eLife, Feb 4, 2019; DOI: 10.7554/eLife.41124

TEER measurement

Authors: Lingjun Tong, Huaxi Yi and Jiong-Wei Wang
Date: Jun 20, 2025，Views：309

Original protocol used in research article：

Milk-derived extracellular vesicles protect intestinal barrier integrity in the gut-liver axis
Science Advances, Apr 12, 2023; DOI: 10.1126/sciadv.ade5041

MD simulations

Author: Pierre Cazade
Date: Jun 20, 2025，Views：17

Original protocol used in research article：

Modulating the pro-apoptotic activity of cytochrome c at a biomimetic electrified interface
Science Advances, Nov 5, 2021; DOI: 10.1126/sciadv.abg4119

Generation of CRISPR/Cas9-induced BMD mice

Authors: Daigo Miyazaki, Mitsuto Sato, Naoko Shiba, Takahiro Yoshizawa and Akinori Nakamura
Date: Jun 18, 2025，Views：349

Original protocol used in research article：

Becker muscular dystrophy mice showed site-specific decay of type IIa fibers with capillary change in skeletal muscle
eLife, Mar 17, 2025; DOI: 10.7554/eLife.100665

2-NBDG uptake

Author: Xiaoxia Liu
Date: Jun 13, 2025，Views：28

Original protocol used in research article：

Metabolomics reveals that CAF-derived lipids promote colorectal cancer peritoneal metastasis by enhancing membrane fluidity
International Journal of Biological Sciences, Feb 21, 2022; DOI: 10.7150/ijbs.68484

Celsr1 Immunostaining

Authors: Danelle Devenport and Stahley N Sara
Date: Jun 9, 2025，Views：356

Original protocol used in research article：

Celsr1 adhesive interactions mediate the asymmetric organization of planar polarity complexes
eLife, Feb 2, 2021; DOI: 10.7554/eLife.62097

Culture of MutuDC cell line

Authors: Kirsteen M Tullett and Mireille H Lahoud
Date: Jun 5, 2025，Views：378

Original protocol used in research article：

RNF41 regulates the damage recognition receptor Clec9A and antigen cross-presentation in mouse dendritic cells
eLife, Dec 2, 2020; DOI: 10.7554/eLife.63452

Detection of glycolipids in plasma membrane fractions

Authors: Alam García-Heredia, Takehiro Kado, Julia Puffal, Yasu S Morita and M Sloan Siegrist
Date: Jun 5, 2025，Views：363

Original protocol used in research article：

Membrane-partitioned cell wall synthesis in mycobacteria
eLife, Feb 5, 2021; DOI: 10.7554/eLife.60263

Construction of plasmids

Author: Qingquan Xiao
Date: Jun 4, 2025，Views：350

Original protocol used in research article：

Rescue of autosomal dominant hearing loss by in vivo delivery of mini dCas13X-derived RNA base editor
Science Translational Medicine, Jul 20, 2022; DOI: 10.1126/scitranslmed.abn0449

Sample processing

Authors: Silvia Portugal and Peter D Crompton
Date: May 23, 2025，Views：383

Original protocol used in research article：

Malaria-associated atypical memory B cells exhibit markedly reduced B cell receptor signaling and effector function
eLife, May 8, 2015; DOI: 10.7554/eLife.07218

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