Bio-101 - Improve Research Reproducibility

Isolation of mouse footpad cells for immunophenotyping

Authors: Anthony Torres-Ruesta, Laurent Renia and Lisa FP Ng
Date: Jul 12, 2022，Views：649

Original protocol used in research article：

Malaria abrogates O’nyong–nyong virus pathologies by restricting virus infection in nonimmune cells
Life Science Alliance, Jan 17, 2022; DOI: 10.26508/lsa.202101272

Ex vivo organotypic cerebellum slice culture (OCSC)

Authors: Charles Capdeville and Martin Baumgartner
Date: Jul 12, 2022，Views：583

Original protocol used in research article：

Spatial proteomics finds CD155 and Endophilin-A1 as mediators of growth and invasion in medulloblastoma
Life Science Alliance, Mar 16, 2022; DOI: 10.26508/lsa.202201380

Western blotting analysis

Authors: Nan Lu, Chengsheng Zhang and Cai Zhang
Date: Jul 11, 2022，Views：557

Original protocol used in research article：

STING agonist cGAMP enhances anti-tumor activity of CAR-NK cells against pancreatic cancer
Oncoimmunology, Mar 21, 2022; DOI: 10.1080/2162402X.2022.2054105

Insulin stimulation assays

Authors: Max Friesen and Rudolf Jaenisch
Date: Jul 11, 2022，Views：614

Original protocol used in research article：

Development of a physiological insulin resistance model in human stem cell–derived adipocytes
Science Advances, Jun 17, 2022; DOI: 10.1126/sciadv.abn7298

Cytokines, chemokines, and AnxA1 quantification

Authors: Mauro Martins Teixeira, Mauro Perretti, Vivian Vasconcelos Costa and Michelle A Sugimoto
Date: Jul 11, 2022，Views：565

Original protocol used in research article：

Targeting the Annexin A1-FPR2/ALX pathway for host-directed therapy in dengue disease
eLife, Mar 16, 2022; DOI: 10.7554/eLife.73853

Single nuclear capture, sequencing, and data analysis

Authors: Minh Q Nguyen, Nicholas Ryba and Steve Davidson
Date: Jul 11, 2022，Views：636

Original protocol used in research article：

Single-nucleus transcriptomic analysis of human dorsal root ganglion neurons
eLife, Nov 26, 2021; DOI: 10.7554/eLife.71752

Validation and Survival Analysis of Hub Genes

Authors: Yanan Wang and Jiguang Guo
Date: Jul 10, 2022，Views：533

Original protocol used in research article：

Identification of Down-Regulated ADH1C is Associated With Poor Prognosis in Colorectal Cancer Using Bioinformatics Analysis
Frontiers in Molecular Biosciences, Mar 1, 2022; DOI: 10.3389/fmolb.2022.791249

DNA Extraction and Sequencing

Authors: Changquan Zhang and Yadong Guo
Date: Jul 10, 2022，Views：572

Original protocol used in research article：

Characterization and Comparative Analysis of Mitochondrial Genomes Among the Calliphoridae (Insecta: Diptera: Oestroidea) and Phylogenetic Implications
Frontiers in Genetics, Feb 17, 2022; DOI: 10.3389/fgene.2022.799203

Coulter counter measurement

Authors: Douaa Mugahid and Marc W Kirschner
Date: Jul 10, 2022，Views：558

Original protocol used in research article：

YAP regulates cell size and growth dynamics via non-cell autonomous mediators
eLife, Jan 9, 2020; DOI: 10.7554/eLife.53404

Immunofluorescence microscopy and analysis

Authors: Michèle MG Hillege, Andi Shi and Richard T Jaspers
Date: Jul 10, 2022，Views：549

Original protocol used in research article：

Lack of Tgfbr1 and Acvr1b synergistically stimulates myofibre hypertrophy and accelerates muscle regeneration
eLife, ; DOI: 10.7554/eLife.77610

First
Prev

Next
Last

Protocols 

Request a Protocol

Receive Our Alerts

Submit 

About