Protocols shared by authors via RaP service
Journal
Yeast toxicity assays
Authors: Zhao-Qing Luo and Jiaqi Fu
Date: Sep 8, 2022，Views：561
Original protocol used in research article：
Legionella pneumophila modulates host energy metabolism by ADP-ribosylation of ADP/ATP translocases
eLife, Jan 27, 2022; DOI: 10.7554/eLife.73611
Crystal violet assay
Authors: Lisanne de Vor and Suzan HM Rooijakkers
Date: Sep 8, 2022，Views：1177
Original protocol used in research article：
Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm
eLife, Jan 6, 2022; DOI: 10.7554/eLife.67301
Single-cell/-nucleus processing for sc/snRNA-seq
Authors: Rebecca D Hodge and Bosiljka Tasic
Date: Sep 7, 2022，Views：621
Original protocol used in research article：
Single-cell and single-nucleus RNA-seq uncovers shared and distinct axes of variation in dorsal LGN neurons in mice, non-human primates, and humans
eLife, Sep 2, 2021; DOI: 10.7554/eLife.64875
Measures
Author: Andrew S. Huhn
Date: Sep 7, 2022，Views：531
Original protocol used in research article：
Suvorexant ameliorated sleep disturbance, opioid withdrawal, and craving during a buprenorphine taper
Science Translational Medicine, Jun 22, 2022; DOI: 10.1126/scitranslmed.abn8238
CytoTRACE analysis in the mammary epithelial atlas paper (https://doi.org/10.1038/s42003-021-02201-2)
Authors: Kohei Saeki and Shiuan Chen
Date: Sep 6, 2022，Views：540
Original protocol used in research article：
Mammary cell gene expression atlas links epithelial cell remodeling events to breast carcinogenesis
Communications Biology, Jun 2, 2021; DOI: 10.1038/s42003-021-02201-2
Craniofacial morphology by micro-CT
Authors: Nandini Singh and Roger H Reeves
Date: Sep 6, 2022，Views：565
Original protocol used in research article：
A non-mosaic transchromosomic mouse model of Down syndrome carrying the long arm of human chromosome 21
eLife, Jun 29, 2020; DOI: 10.7554/eLife.56223
Mouse tissue collection and assessments
Authors: Dominik Saul, Joshua N Farr and Sundeep Khosla
Date: Sep 6, 2022，Views：570
Original protocol used in research article：
Modulation of fracture healing by the transient accumulation of senescent cells
eLife, Oct 7, 2021; DOI: 10.7554/eLife.69958
Human erythrocyte hemolysis assay
Authors: Aftab Nadeem and Sun Nyunt Wai
Date: Sep 6, 2022，Views：750
Original protocol used in research article：
Protein-lipid interaction at low pH induces oligomerization of the MakA cytotoxin from Vibrio cholerae
eLife, Feb 8, 2022; DOI: 10.7554/eLife.73439
Covariates for GLM
Authors: Katy AM Gaythorpe and Neil Ferguson
Date: Sep 5, 2022，Views：565
Original protocol used in research article：
The global burden of yellow fever
eLife, Mar 16, 2021; DOI: 10.7554/eLife.64670
KCC2 measurements and analysis of subcellular distribution
Authors: Franck Aby and Pascal Fossat
Date: Sep 5, 2022，Views：563
Original protocol used in research article：
Switch of serotonergic descending inhibition into facilitation by a spinal chloride imbalance in neuropathic pain
Science Advances, Jul 27, 2022; DOI: 10.1126/sciadv.abo0689
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