Protocols shared by authors via RaP service
Journal
Sample preparation for FLAG pull down
Authors: Donghai Wang, Hudan Liu, Kaiwei Liang and Guoliang Qing
Date: Jul 10, 2024，Views：85
Original protocol used in research article：
The super elongation complex drives transcriptional addiction in MYCN-amplified neuroblastoma
Science Advances, Mar 29, 2023; DOI: 10.1126/sciadv.adf0005
Tonoplast membrane preparation
Authors: Upendo Lupanga and Karin Schumacher
Date: Jul 9, 2024，Views：53
Original protocol used in research article：
The Arabidopsis V-ATPase is localized to the TGN/EE via a seed plant-specific motif
eLife, Nov 25, 2020; DOI: 10.7554/eLife.60568
Mouse B cell culture
Authors: Roberta Buono and David A. Fruman
Date: Jul 1, 2024，Views：93
Original protocol used in research article：
Reduced eIF4E function impairs B-cell leukemia without altering normal B-lymphocyte function
iScience, Jun 17, 2021; DOI: 10.1016/j.isci.2021.102748
How to make anti-IgA beads for serum IgA purification
Authors: Yoshihito Nihei and Daisuke Kitamura
Date: Jun 25, 2024，Views：85
Original protocol used in research article：
Identification of IgA autoantibodies targeting mesangial cells redefines the pathogenesis of IgA nephropathy
Science Advances, Mar 22, 2023; DOI: 10.1126/sciadv.add6734
RiboPuromycylation
Authors: Sangeetha Devi Kumar and Sandeep M Eswarappa
Date: Jun 22, 2024，Views：49
Original protocol used in research article：
Evidence for low-level translation in human erythrocytes
Molecular Biology of the Cell, Sep 22, 2022; DOI: 10.1091/mbc.E21-09-0437
RNA pull-down assay
Authors: Chuannan Fan and Peter ten Dijke
Date: Jun 13, 2024，Views：14
Original protocol used in research article：
The lncRNA LETS1 promotes TGF-β–induced EMT and cancer cell migration by transcriptionally activating a TβR1-stabilizing mechanism
SCIENCE SIGNALING, Jun 20, 2023; DOI: 10.1126/scisignal.adf1947
Intracytoplasmic sperm injection（ICSI）
Authors: Yongliang Shang, Fei Gao, Xuejiang Guo, Qing-Yuan Sun, Yunxia Cao and Wei Li
Date: Jun 7, 2024，Views：374
Original protocol used in research article：
Essential role for SUN5 in anchoring sperm head to the tail
eLife, Sep 25, 2017; DOI: 10.7554/eLife.28199
Biochemical measurement of Rab5 activity
Author: Muralidharan Mani
Date: Jun 4, 2024，Views：272
Original protocol used in research article：
Developmentally regulated GTP-binding protein 2 coordinates Rab5 activity and transferrin recycling
Molecular Biology of the Cell, Jan 15, 2016; DOI: 10.1091/mbc.E15-08-0558
RNA isolation, cDNA synthesis, and quantitative RT-PCR
Authors: Keshav Thapa and Petteri Rinne
Date: Jun 4, 2024，Views：547
Original protocol used in research article：
Melanocortin 1 receptor regulates cholesterol and bile acid metabolism in the liver
eLife, Jul 25, 2023; DOI: 10.7554/eLife.84782
Mouse intestinal epithelial cell strip
Authors: Timothy J Nice and David A Constant
Date: May 24, 2024，Views：540
Original protocol used in research article：
Homeostatic interferon-lambda response to bacterial microbiota stimulates preemptive antiviral defense within discrete pockets of intestinal epithelium
eLife, Feb 9, 2022; DOI: 10.7554/eLife.74072
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.