Protocols shared by authors via RaP service
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Preparation of acute brain slice for ex vivo time-lapse imaging
Authors: Sunitha Subhramanian and Ilia V Baskakov
Date: Feb 5, 2026，Views：16
Original protocol used in research article：
Dissecting surveying behavior of reactive microglia under chronic neurodegeneration
eLife, Jan 8, 2026; DOI: 10.7554/eLife.107650
Determination of BV2 Microglial Phagocytic Activity (Fluorescent Microspheres / FluoSpheres Assay; Flow Cytometry/Microscopy)
Authors: Marta Matuszewska, Magdalena Cieślik, Anna Wilkaniec and Grzegorz A. Czapski
Date: Feb 5, 2026，Views：9
Original protocol used in research article：
The Inhibition of Bromodomain and Extraterminal Domain (BET) Proteins Protects Against Microglia-Mediated Neuronal Loss In Vitro
Biomolecules, Apr 4, 2025; DOI: 10.3390/biom15040528
NLRP3 oligomerization
Authors: Xiao-Ming Yang and Rong-Hua Yin
Date: Feb 3, 2026，Views：3
Original protocol used in research article：
Pharmacological targeting of NLRP3 deubiquitination for treatment of NLRP3-associated inflammatory diseases
Science Immunology, Apr 29, 2021; DOI: 10.1126/sciimmunol.abe2933
Production and usage of VSV-G pseudotyped lentiviruses
Authors: Soham P. Chowdhury, Diego Acosta-Alvear and Francesca Zappa
Date: Jan 31, 2026，Views：19
Original protocol used in research article：
Baseline unfolded protein response signaling adjusts the timing of the mammalian cell cycle
Molecular Biology of the Cell, May 20, 2024; DOI: 10.1091/mbc.E23-11-0419
Immunofluorescence
Authors: Irene Bolea and Albert Quintana
Date: Jan 28, 2026，Views：36
Original protocol used in research article：
Defined neuronal populations drive fatal phenotype in a mouse model of Leigh syndrome
eLife, Aug 12, 2019; DOI: 10.7554/eLife.47163
3.8. Ethidium Bromide Displacement Fluorescence Assay
Author: Francesco Ruffo
Date: Jan 27, 2026，Views：3
Original protocol used in research article：
Square-Planar vs. Trigonal Bipyramidal Geometry in Pt(II) Complexes Containing Triazole-Based Glucose Ligands as Potential Anticancer Agents
International Journal of Molecular Sciences, Aug 13, 2021; DOI: 10.3390/ijms22168704
Co-immunoprecipitation (co-IP) Following SUMOylation/Ubiquitination Perturbation
Authors: Seung Han Son and Chul Geun Kim
Date: Jan 26, 2026，Views：35
Original protocol used in research article：
SUMOylation-mediated PSME3-20S proteasomal degradation of transcription factor CP2c is crucial for cell cycle progression
Science Advances, Jan 27, 2023; DOI: 10.1126/sciadv.add4969
Enrichr pathway analysis: Reactome (2022) and Gene Ontology (GO Biological Processes 2023) bulk RNA and luminal proteomics analysis
Authors: Ryan Finnerty and Wipawee Winuthayanon
Date: Jan 23, 2026，Views：37
Original protocol used in research article：
Multi-omics analyses and machine learning prediction of oviductal responses in the presence of gametes and embryos
eLife, Feb 26, 2025; DOI: 10.7554/eLife.100705
Gene Set enrichment analysis
Authors: Hannah R Monday and Pablo Castillo
Date: Jan 14, 2026，Views：45
Original protocol used in research article：
CB1-receptor-mediated inhibitory LTD triggers presynaptic remodeling via protein synthesis and ubiquitination
eLife, Sep 9, 2020; DOI: 10.7554/eLife.54812
Culture conditions for new cell lines
Authors: Norbert Perrimon and Amanda Simcox
Date: Jan 9, 2026，Views：80
Original protocol used in research article：
Continuous muscle, glial, epithelial, neuronal, and hemocyte cell lines for Drosophila research
eLife, Jul 20, 2023; DOI: 10.7554/eLife.85814
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