Bio-101 - Improve Research Reproducibility

Preparation of nano-Ag@erythrosomes

Authors: Zhuang Liu, Zhen Gu and Chao Wang
Date: Aug 22, 2022，Views：552

Original protocol used in research article：

Red blood cell–derived nanoerythrosome for antigen delivery with enhanced cancer immunotherapy
Science Advances, Oct 23, 2019; DOI: 10.1126/sciadv.aaw6870

PCR analysis of the targeting events for HDR, NHEJ, and multiple copy integration

Authors: Boris V. Skryabin, Timofey S. Rozhdestvensky and Juergen Brosius
Date: Aug 19, 2022，Views：540

Original protocol used in research article：

Pervasive head-to-tail insertions of DNA templates mask desired CRISPR-Cas9–mediated genome editing events
Science Advances, Feb 11, 2020; DOI: 10.1126/sciadv.aax2941

scrambled RNPs

Authors: François Kroll and Jason Rihel
Date: Aug 19, 2022，Views：634

Original protocol used in research article：

A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes
eLife, Jan 8, 2021; DOI: 10.7554/eLife.59683

Population structure and signatures of selection

Author: Luke Thomas
Date: Aug 18, 2022，Views：541

Original protocol used in research article：

Spatially varying selection between habitats drives physiological shifts and local adaptation in a broadcast spawning coral on a remote atoll in Western Australia
Science Advances, Apr 27, 2022; DOI: 10.1126/sciadv.abl9185

Does TRH increase LSNA or BAT SNA via actions in the DMH and/or RPa?

Authors: Zhigang Shi, Daniel L Marks and Virginia L Brooks
Date: Aug 18, 2022，Views：547

Original protocol used in research article：

Leptin increases sympathetic nerve activity via induction of its own receptor in the paraventricular nucleus
eLife, Jun 15, 2020; DOI: 10.7554/eLife.55357

FtsZ and GpsB purification

Authors: Prahathees J Eswara and Kumaran S Ramamurthi
Date: Aug 18, 2022，Views：573

Original protocol used in research article：

An essential Staphylococcus aureus cell division protein directly regulates FtsZ dynamics
eLife, Oct 2, 2018; DOI: 10.7554/eLife.38856

TraDIS method.

Authors: Karthik Pullela, Ian R. Henderson, Alessandra Polissi and Waldemar Vollmer
Date: Aug 17, 2022，Views：538

Original protocol used in research article：

DpaA Detaches Braun’s Lipoprotein from Peptidoglycan
mBio, May 4, 2021; DOI: 10.1128/mbio.00836-21

In vitro culture on tissue culture plastic, fibroblasts, or keratinocytes

Authors: Tiago R. Matos, Jessica E. Teague and Rachael A. Clark
Date: Aug 16, 2022，Views：595

Original protocol used in research article：

Central memory T cells are the most effective precursors of resident memory T cells in human skin
Science Immunology, Apr 22, 2022; DOI: 10.1126/sciimmunol.abn1889

Diversity based on T cell composition

Authors: Liangtao Zheng, Shishang Qin, Xueda Hu and Zemin Zhang
Date: Aug 15, 2022，Views：597

Original protocol used in research article：

Pan-cancer single-cell landscape of tumor-infiltrating T cells
Science, Dec 17, 2021; DOI: 10.1126/science.abe6474

Indisulam efficacy studies in in vivo models

Authors: Shivendra Singh, Waise Quarni, Deepak Nijhawan, Andrew M. Davidoff and Jun Yang
Date: Aug 15, 2022，Views：555

Original protocol used in research article：

Targeting the spliceosome through RBM39 degradation results in exceptional responses in high-risk neuroblastoma models
Science Advances, Nov 17, 2021; DOI: 10.1126/sciadv.abj5405

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