Protocols shared by authors via RaP service
Journal
2.5. Stool Bacterial Extracellular Vesicle Extraction and Removal of Exosomes
Authors: Chanel A. Mosby and Melissa K. Jones
Date: Nov 10, 2025，Views：590
Original protocol used in research article：
Changes in the Murine Microbiome and Bacterial Extracellular Vesicle Production in Response to Antibiotic Treatment and Norovirus Infection
Viruses, Dec 16, 2023; DOI: 10.3390/v15122443
Identification of apoptotic and necrotic cells
Author: H. de Cock
Date: Nov 10, 2025，Views：8
Original protocol used in research article：
Variation of virulence of five Aspergillus fumigatus isolates in four different infection models
PLoS ONE, Jul 9, 2021; DOI: 10.1371/journal.pone.0252948
Histochemical GUS analysis in transgenic rice
Authors: Mitra Mazarei and Charles Neal Stewart Jr.
Date: Nov 7, 2025，Views：591
Original protocol used in research article：
Switchgrass (Panicum virgatum L.) promoters for green tissue-specific expression of the MYB4 transcription factor for reduced-recalcitrance transgenic switchgrass
Biotechnology for Biofuels, Apr 24, 2018; DOI: 10.1186/s13068-018-1119-7
CitH3‐DNA ELISA
Authors: Yuanlin Song, Qiaoliang Zhu and Jian Zhou
Date: Nov 2, 2025，Views：24
Original protocol used in research article：
Lianhua Qingke ameliorates lipopolysaccharide‐induced lung injury by inhibiting neutrophil extracellular traps formation and pyroptosis
Pulmonary Circulation, Oct 6, 2023; DOI: 10.1002/pul2.12295
Genotyping CCR7-flox mice
Author: Milena Bogunovic
Date: Oct 27, 2025，Views：10
Original protocol used in research article：
Gut-resident CX3CR1hi macrophages induce tertiary lymphoid structures and IgA response in situ
Science Immunology, Apr 9, 2020; DOI: 10.1126/sciimmunol.aax0062
Organoid Seeding Protocol on Chips for MEA recordings
Authors: Michele Bertacchi and Michèle Studer
Date: Oct 26, 2025，Views：591
Original protocol used in research article：
FGF8-mediated gene regulation affects regional identity in human cerebral organoids
eLife, Nov 1, 2024; DOI: 10.7554/eLife.98096
Sample preparation for light sheet microscopy
Authors: Johannes Krug, Christoph Englert, Birgit Perner, Carolin Albertz, Hanna Mörl Mörl and Vera Hopfenmüller
Date: Oct 17, 2025，Views：251
Original protocol used in research article：
Generation of a transparent killifish line through multiplex CRISPR/Cas9mediated gene inactivation
eLife, Feb 23, 2023; DOI: 10.7554/eLife.81549
In vivo light sheet imaging
Authors: Johannes Krug, Christoph Englert, Birgit Perner, Carolin Albertz, Hanna Mörl Mörl and Vera Hopfenmüller
Date: Oct 17, 2025，Views：24
Original protocol used in research article：
Generation of a transparent killifish line through multiplex CRISPR/Cas9mediated gene inactivation
eLife, Feb 23, 2023; DOI: 10.7554/eLife.81549
Fluorescence-based ion transport assays using Proteoliposomes
Authors: Cristina Manatschal and Karthik Ramanadane
Date: Sep 20, 2025，Views：315
Original protocol used in research article：
Structural and functional properties of a magnesium transporter of the SLC11/NRAMP family
eLife, Jan 10, 2022; DOI: 10.7554/eLife.74589
Mounting
Authors: John Darby Cole and Sebastian Jessberger
Date: Sep 18, 2025，Views：183
Original protocol used in research article：
Characterization of the neurogenic niche in the aging dentate gyrus using iterative immunofluorescence imaging
eLife, Jan 28, 2022; DOI: 10.7554/eLife.68000
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