Bio-101 - Improve Research Reproducibility

ERCC spike-in normalized mRNA-seq for cultured cells (human)

Authors: Mark W. Zimmerman, Brian J. Abraham and A. Thomas Look
Date: Jun 1, 2022，Views：669

Original protocol used in research article：

Retinoic acid rewires the adrenergic core regulatory circuitry of childhood neuroblastoma
Science Advances, Oct 20, 2021; DOI: 10.1126/sciadv.abe0834

Western blot assay

Authors: Chenkui Miao and Li Jia
Date: May 30, 2022，Views：652

Original protocol used in research article：

RB1 loss overrides PARP inhibitor sensitivity driven by RNASEH2B loss in prostate cancer
Science Advances, Feb 18, 2022; DOI: 10.1126/sciadv.abl9794

Test for tannins (ferric chloride test)

Authors: Dereje Nigussie and Dereje Nigussie
Date: May 30, 2022，Views：3457

Original protocol used in research article：

Antibacterial activity of methanol extracts of the leaves of three medicinal plants against selected bacteria isolated from wounds of lymphoedema patients
BMC Complementary Medicine and Therapies, Jan 3, 2021; DOI: 10.1186/s12906-020-03183-0

10x genomics

Authors: Sudhir Gopal Tattikota and Norbert Perrimon
Date: May 27, 2022，Views：654

Original protocol used in research article：

A single-cell survey of Drosophila blood
eLife, May 12, 2020; DOI: 10.7554/eLife.54818

Transfections of poly(I:C), DNA, miRNA mimics and Antagomirs

Authors: Jeroen Witteveldt and Sara Macias
Date: May 27, 2022，Views：2374

Original protocol used in research article：

MicroRNA-deficient mouse embryonic stem cells acquire a functional interferon response
eLife, Apr 23, 2019; DOI: 10.7554/eLife.44171

SOD Activity

Authors: Swetha Sudha Nagamalla and Someswar Rao Sagurthi
Date: May 26, 2022，Views：1035

Original protocol used in research article：

Morpho-Physiological and Proteomic Response of Bt-Cotton and Non-Bt Cotton to Drought Stress
Frontiers in Plant Science, May 10, 2021; DOI: 10.3389/fpls.2021.663576

Generation of Genotypes

Authors: Jose Alquicira-Hernandez, Kristof Wing, Alex W. Hewitt and Joseph E. Powell
Date: May 26, 2022，Views：694

Original protocol used in research article：

Single-cell eQTL mapping identifies cell type–specific genetic control of autoimmune disease
Science, Apr 8, 2022; DOI: 10.1126/science.abf3041

ChIP-seq and RNA-seq

Author: Jia-Ray Yu
Date: May 26, 2022，Views：684

Original protocol used in research article：

The H3K36me2 writer-reader dependency in H3K27M-DIPG
Science Advances, Jul 14, 2021; DOI: 10.1126/sciadv.abg7444

Measuring resistance-infectivity (co)evolution

Author: Meaghan Castledine
Date: May 26, 2022，Views：641

Original protocol used in research article：

Parallel evolution of Pseudomonas aeruginosa phage resistance and virulence loss in response to phage treatment in vivo and in vitro
eLife, Feb 21, 2022; DOI: 10.7554/eLife.73679

Datasets used in this study

Authors: Jie Lyu, Jingyi Jessica Li and Wei Li
Date: May 25, 2022，Views：593

Original protocol used in research article：

DORGE: Discovery of Oncogenes and tumoR suppressor genes using Genetic and Epigenetic features
Science Advances, Nov 11, 2020; DOI: 10.1126/sciadv.aba6784

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