Protocols shared by authors via RaP service
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Authors: Madeline Cassani and Geraldine SEYDOUX
Date: Jun 18, 2022，Views：625
Original protocol used in research article：
Puromycin reactivity does not accurately localize translation at the subcellular level
eLife, Aug 26, 2020; DOI: 10.7554/eLife.60303
Immunofluorescence and analyses of PB number and ER-PB colocalization
Authors: Jason E. Lee and Gia K. Voeltz
Date: Jun 18, 2022，Views：582
Original protocol used in research article：
Endoplasmic reticulum contact sites regulate the dynamics of membraneless organelles
Science, Jan 30, 2020; DOI: 10.1126/science.aay7108
In vivo cell depletion
Authors: Michiyuki Matsuda and Hiroshi Ichise
Date: Jun 18, 2022，Views：591
Original protocol used in research article：
Functional visualization of NK cell-mediated killing of metastatic single tumor cells
eLife, Feb 3, 2022; DOI: 10.7554/eLife.76269
Detection of SARS-CoV-2 specific IgG and IgM antibodies
Authors: Ling Wang and Zhi Q. Yao
Date: Jun 17, 2022，Views：548
Original protocol used in research article：
Blockade of SARS-CoV-2 spike protein-mediated cell–cell fusion using COVID-19 convalescent plasma
Scientific Reports, Mar 10, 2021; DOI: 10.1038/s41598-021-84840-3
A2AR expression, purification, and nanodisc reconstitution
Authors: Shuya Kate Huang and R Scott Prosser
Date: Jun 15, 2022，Views：725
Original protocol used in research article：
Allosteric modulation of the adenosine A2A receptor by cholesterol
eLife, Jan 5, 2022; DOI: 10.7554/eLife.73901
Establishing Endometrial Assembloids to Model Human Embryo Implantation in vitro
Authors: Thomas M Rawlings, Maria Tryfonos, Komal Makwana, Deborah M Taylor, Jan J Brosens and Emma S Lucas
Date: Jun 15, 2022，Views：891
Original protocol used in research article：
Modelling the impact of decidual senescence on embryo implantation in human endometrial assembloids
eLife, Sep 6, 2021; DOI: 10.7554/eLife.69603
The Therapeutic Factors Inventory-19 (TFI-19)
Authors: Nicholas K. Canby and Kristina Eichel
Date: Jun 14, 2022，Views：683
Original protocol used in research article：
The Contribution of Common and Specific Therapeutic Factors to Mindfulness-Based Intervention Outcomes
Frontiers in Psychology, Jan 14, 2021; DOI: 10.3389/fpsyg.2020.603394
Urine-based Enzyme-Linked Immunosorbent Assay to detect anti-SARS-CoV-2 nucleocapsid protein antibodies
Authors: Fernanda Ludolf, Fernanda F Ramos, Flávia Bagno, Eduardo A F Coelho and Flavio G Fonseca
Date: Jun 14, 2022，Views：759
Original protocol used in research article：
Detecting anti–SARS-CoV-2 antibodies in urine samples: A noninvasive and sensitive way to assay COVID-19 immune conversion
Science Advances, May 13, 2022; DOI: 10.1126/sciadv.abn7424
Ribosome profiling with retapamulin treatment (Ribo-RET)
Authors: Keith M Derbyshire, Todd A Gray and Joseph T Wade
Date: Jun 13, 2022，Views：614
Original protocol used in research article：
Pervasive translation in Mycobacterium tuberculosis
eLife, ; DOI: 10.7554/eLife.73980
Plasmids and lentivirus production
Authors: Diljeet Gill, Inês Milagre and Wolf Reik
Date: Jun 13, 2022，Views：891
Original protocol used in research article：
Multi-omic rejuvenation of human cells by maturation phase transient reprogramming
eLife, Apr 8, 2022; DOI: 10.7554/eLife.71624
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