Bio-101 - Improve Research Reproducibility

In vivo muscle regeneration and cross-sectional immunofluorescence

Authors: Claudia Dominici and Stéphane Richard
Date: Jul 5, 2022，Views：558

Original protocol used in research article：

Muscle stem cell polarity requires QKI-mediated alternative splicing of Integrin Alpha-7 (Itga7)
Life Science Alliance, Feb 14, 2022; DOI: 10.26508/lsa.202101192

Cell suspension preparation for flow cytometry and flow cytometry analysis

Authors: Nehemiah Cox and Frederic Geissmann
Date: Jul 5, 2022，Views：568

Original protocol used in research article：

Diet-regulated production of PDGFcc by macrophages controls energy storage
Science, Jul 2, 2021; DOI: 10.1126/science.abe9383

Thioflavin S staining

Authors: Zengqiang Yuan, Rui-Yuan Pan and Zengqiang Yuan
Date: Jul 1, 2022，Views：635

Original protocol used in research article：

Sodium rutin ameliorates Alzheimer’s disease–like pathology by enhancing microglial amyloid-β clearance
Science Advances, Feb 27, 2019; DOI: 10.1126/sciadv.aau6328

Cell culture and transfection

Authors: Frank Buchholz and Li Ding
Date: Jul 1, 2022，Views：1095

Original protocol used in research article：

DNA methylation–independent long-term epigenetic silencing with dCRISPR/Cas9 fusion proteins
Life Science Alliance, Mar 14, 2022; DOI: 10.26508/lsa.202101321

Oxygen-glucose deprivation (OGD)

Authors: Miren Revuelta and Thomas Schmitz
Date: Jun 30, 2022，Views：733

Original protocol used in research article：

In vitro P38MAPK inhibition in aged astrocytes decreases reactive astrocytes, inflammation and increases nutritive capacity after oxygen-glucose deprivation
Aging (Albany NY), Feb 9, 2021; DOI: 10.18632/aging.202651

Total RNA extraction from fish fin ray

Author: Mari Sato
Date: Jun 29, 2022，Views：593

Original protocol used in research article：

Osteocytes as main responders to low-intensity pulsed ultrasound treatment during fracture healing
Scientific Reports, May 13, 2021; DOI: 10.1038/s41598-021-89672-9

Phylogenetic distance analysis and clustering

Author: Ricardo A. Segovia
Date: Jun 29, 2022，Views：569

Original protocol used in research article：

Freezing and water availability structure the evolutionary diversity of trees across the Americas
Science Advances, May 5, 2020; DOI: 10.1126/sciadv.aaz5373

Cell proliferation assay

Authors: Linhong Deng, Allen P. Liu and Mingzhi Luo
Date: Jun 29, 2022，Views：581

Original protocol used in research article：

Compression enhances invasive phenotype and matrix degradation of breast Cancer cells via Piezo1 activation
BMC Molecular and Cell Biology, Jan 3, 2022; DOI: 10.1186/s12860-021-00401-6

Study design, inclusion and exclusion criteria.

Authors: Riccardo Pofi, Elisa Giannetta, Daniele Gianfrilli and Andrea M. Isidori
Date: Jun 29, 2022，Views：549

Original protocol used in research article：

Sex-specific effects of daily tadalafil on diabetic heart kinetics in RECOGITO, a randomized, double-blind, placebo-controlled trial
Science Translational Medicine, Jun 15, 2022; DOI: 10.1126/scitranslmed.abl8503

IVDi culture

Authors: Takashi Yoshino and Katsuhiko Hayashi
Date: Jun 28, 2022，Views：613

Original protocol used in research article：

Generation of ovarian follicles from mouse pluripotent stem cells
Science, Jul 16, 2021; DOI: 10.1126/science.abe0237

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