Bio-101 - Improve Research Reproducibility

Permutation test

Authors: Guan Ning Lin, Weichen Song and Zhen Wang
Date: Jun 7, 2022，Views：581

Original protocol used in research article：

De novo mutations identified by whole-genome sequencing implicate chromatin modifications in obsessive-compulsive disorder
Science Advances, Jan 12, 2022; DOI: 10.1126/sciadv.abi6180

Total RNA-seq

Authors: Ane Martin Anduaga and Sebastian Kadener
Date: Jun 6, 2022，Views：597

Original protocol used in research article：

Thermosensitive alternative splicing senses and mediates temperature adaptation in Drosophila
eLife, Nov 8, 2019; DOI: 10.7554/eLife.44642

Drosophila S2 cells RNA extraction

Authors: Subhendu Roy Choudhury and Saverio Brogna
Date: Jun 5, 2022，Views：636

Original protocol used in research article：

Exon junction complex proteins bind nascent transcripts independently of pre-mRNA splicing in Drosophila melanogaster
eLife, Nov 23, 2016; DOI: 10.7554/eLife.19881

Translation efficiency calculation

Authors: David H Burkhardt, Silvi Rouskin, Gene-Wei Li, Jonathan S Weissman, Carol A Gross and Yan Zhang
Date: Jun 3, 2022，Views：903

Original protocol used in research article：

Operon mRNAs are organized into ORF-centric structures that predict translation efficiency
eLife, Jan 31, 2017; DOI: 10.7554/eLife.22037

Immunofluorescence

Authors: Ying Ying Yiu, Grace Blacker and Irving L. Weissman
Date: Jun 2, 2022，Views：611

Original protocol used in research article：

CD47 Blockade Leads to Chemokine-Dependent Monocyte Infiltration and Loss of B Cells from the Splenic Marginal Zone
J Immunol, Mar 15, 2022; DOI: 10.4049/jimmunol.2100352

ERCC spike-in normalized mRNA-seq for cultured cells (human)

Authors: Mark W. Zimmerman, Brian J. Abraham and A. Thomas Look
Date: Jun 1, 2022，Views：655

Original protocol used in research article：

Retinoic acid rewires the adrenergic core regulatory circuitry of childhood neuroblastoma
Science Advances, Oct 20, 2021; DOI: 10.1126/sciadv.abe0834

Western blot assay

Authors: Chenkui Miao and Li Jia
Date: May 30, 2022，Views：650

Original protocol used in research article：

RB1 loss overrides PARP inhibitor sensitivity driven by RNASEH2B loss in prostate cancer
Science Advances, Feb 18, 2022; DOI: 10.1126/sciadv.abl9794

Test for tannins (ferric chloride test)

Authors: Dereje Nigussie and Dereje Nigussie
Date: May 30, 2022，Views：3374

Original protocol used in research article：

Antibacterial activity of methanol extracts of the leaves of three medicinal plants against selected bacteria isolated from wounds of lymphoedema patients
BMC Complementary Medicine and Therapies, Jan 3, 2021; DOI: 10.1186/s12906-020-03183-0

10x genomics

Authors: Sudhir Gopal Tattikota and Norbert Perrimon
Date: May 27, 2022，Views：651

Original protocol used in research article：

A single-cell survey of Drosophila blood
eLife, May 12, 2020; DOI: 10.7554/eLife.54818

Transfections of poly(I:C), DNA, miRNA mimics and Antagomirs

Authors: Jeroen Witteveldt and Sara Macias
Date: May 27, 2022，Views：2347

Original protocol used in research article：

MicroRNA-deficient mouse embryonic stem cells acquire a functional interferon response
eLife, Apr 23, 2019; DOI: 10.7554/eLife.44171

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