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A high-quality database for basic life science protocols
Protocols
Research fields
Biochemistry (1376)
Biophysics (164)
Cancer Biology (431)
Cell Biology (2122)
Developmental Biology (321)
Immunology (597)
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AIF subcellular localization analysis and determination of AIF pathology score
Authors:
Stephen P. Chelko
and
Nazareno Paolocci
Date: May 16, 2022,Views:605
Original protocol used in research article:
Exercise triggers CAPN1-mediated AIF truncation inducing myocyte cell death in arrhythmogenic cardiomyopathy
Science Translational Medicine, Feb 16, 2021; DOI:
10.1126/scitranslmed.abf0891
Membrane stability index (MSI)
Author:
Natalie De Falco
Date: May 16, 2022,Views:715
Original protocol used in research article:
Geodiversity impacts plant community structure in a semi-arid region
Scientific Reports, Jul 27, 2021; DOI:
10.1038/s41598-021-94698-0
Thermal seizure induction
Author:
Daniel K Mulkey
Date: May 13, 2022,Views:626
Original protocol used in research article:
Disordered breathing in a mouse model of Dravet syndrome
eLife, Apr 26, 2019; DOI:
10.7554/eLife.43387
Single-cell isolations
Author:
Jonathan Kipnis
Date: May 11, 2022,Views:677
Original protocol used in research article:
Skull and vertebral bone marrow are myeloid cell reservoirs for the meninges and CNS parenchyma
Science, Jul 23, 2021; DOI:
10.1126/science.abf7844
Study design
Authors:
Christian Schwartz
and
Padraic G. Fallon
Date: May 10, 2022,Views:579
Original protocol used in research article:
Innate PD-L1 limits T cell–mediated adipose tissue inflammation and ameliorates diet-induced obesity
Science Translational Medicine, Mar 9, 2022; DOI:
10.1126/scitranslmed.abj6879
NPTX2 ELISA assay
Authors:
Paul F Worley
and
Mei-Fang Xiao
Date: May 10, 2022,Views:665
Original protocol used in research article:
NPTX2 and cognitive dysfunction in Alzheimer’s Disease
eLife, Mar 23, 2017; DOI:
10.7554/eLife.23798
Strains, media, and growth conditions
Authors:
Huomiao Ran
and
Wen-Bing Yin
Date: May 10, 2022,Views:628
Original protocol used in research article:
Fungal-fungal cocultivation leads to widespread secondary metabolite alteration requiring the partial loss-of-function VeA1 protein
Science Advances, Apr 27, 2022; DOI:
10.1126/sciadv.abo6094
Measurement of the cADPR levels in DRGs
Authors:
Wanhua Li
,
Hon Cheung Lee
and
Yong Juan Zhao
Date: May 9, 2022,Views:848
Original protocol used in research article:
Permeant fluorescent probes visualize the activation of SARM1 and uncover an anti-neurodegenerative drug candidate
eLife, May 4, 2021; DOI:
10.7554/eLife.67381
Production of environmental sounds
Authors:
Daniel Arthur Abrams
and
Vinod Menon
Date: May 9, 2022,Views:599
Original protocol used in research article:
Impaired voice processing in reward and salience circuits predicts social communication in children with autism
eLife, Feb 26, 2019; DOI:
10.7554/eLife.39906
Plasmid/short hairpin RNA construction and virus infection
Authors:
Yuanyuan Qin
and
Suling Liu
Date: May 8, 2022,Views:648
Original protocol used in research article:
Interfering MSN-NONO complex–activated CREB signaling serves as a therapeutic strategy for triple-negative breast cancer
Science Advances, Feb 19, 2020; DOI:
10.1126/sciadv.aaw9960
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