Bio-101 - Improve Research Reproducibility

Acridine orange/ethidium bromide (AO/EtBr) double staining

Authors: Ahmet Hamdi KEPEKÇİ and Cenk KIG
Date: Jun 13, 2022，Views：1834

Original protocol used in research article：

In Vitro Physiological Effects of Betahistine on Cell Lines of Various Origins
Turkish Journal of Pharmaceutical Sciences, Apr 20, 2021; DOI: 10.4274/tjps.galenos.2020.88155

Single-molecule fluorescence in situ hybridisation (smFISH)

Authors: Jeffrey Y Lee, Peter AC Wing, Alfredo Castello, Jane A Mckeating and Ilan Davis
Date: Jun 13, 2022，Views：648

Original protocol used in research article：

Absolute quantitation of individual SARS-CoV-2 RNA molecules provides a new paradigm for infection dynamics and variant differences
eLife, Jan 20, 2022; DOI: 10.7554/eLife.74153

Mitochondrial OXPHOS efficiency and capacity

Authors: Jeffrey E Pessin and Yan Tang
Date: Jun 12, 2022，Views：562

Original protocol used in research article：

TIGAR deficiency enhances skeletal muscle thermogenesis by increasing neuromuscular junction cholinergic signaling
eLife, Mar 7, 2022; DOI: 10.7554/eLife.73360

Lentivirus infection

Author: Edward R Kastenhuber
Date: Jun 9, 2022，Views：625

Original protocol used in research article：

Coagulation factors directly cleave SARS-CoV-2 spike and enhance viral entry
eLife, Mar 16, 2022; DOI: 10.7554/eLife.77444

Lipid content measurement

Authors: Pan Su, Qiang Wang and Qing Yi
Date: Jun 9, 2022，Views：561

Original protocol used in research article：

Enhanced lipid accumulation and metabolism are required for the differentiation and activation of tumor-associated macrophages
Cancer Res, Feb 3, 2020; DOI: 10.1158/0008-5472.CAN-19-2994

2.11. Glucose uptake and lactate production

Authors: Hui Wang and Xiaoxia Liu
Date: Jun 9, 2022，Views：559

Original protocol used in research article：

Enhancing cancer‐associated fibroblast fatty acid catabolism within a metabolically challenging tumor microenvironment drives colon cancer peritoneal metastasis
Molecular Oncology, Feb 16, 2021; DOI: 10.1002/1878-0261.12917

Negative staining of HIV viral particles permeabilised with PFO with capsid stabilised with IP6

Authors: Derrick Lau, Chantal L Márquez, Michael Parker and Till Böcking
Date: Jun 8, 2022，Views：696

Original protocol used in research article：

Kinetics of HIV-1 capsid uncoating revealed by single-molecule analysis
eLife, Jun 7, 2018; DOI: 10.7554/eLife.34772

Single-cell photoablation of MN2

Author: Kohji Hotta
Date: Jun 8, 2022，Views：637

Original protocol used in research article：

A single motor neuron determines the rhythm of early motor behavior in Ciona
Science Advances, Dec 10, 2021; DOI: 10.1126/sciadv.abl6053

In vitro experiments

Authors: Mark D. Bunting, Maulik Vyas and Shadmehr Demehri
Date: Jun 8, 2022，Views：611

Original protocol used in research article：

Extracellular matrix proteins regulate NK cell function in peripheral tissues
Science Advances, Mar 16, 2022; DOI: 10.1126/sciadv.abk3327

Comparison of mutation severity, gene intolerance, and mutation distribution

Authors: Guan Ning Lin, Weichen Song and Zhen Wang
Date: Jun 7, 2022，Views：602

Original protocol used in research article：

De novo mutations identified by whole-genome sequencing implicate chromatin modifications in obsessive-compulsive disorder
Science Advances, Jan 12, 2022; DOI: 10.1126/sciadv.abi6180

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