Bio-101 - Improve Research Reproducibility

Single nuclear capture, sequencing, and data analysis

Authors: Minh Q Nguyen, Nicholas Ryba and Steve Davidson
Date: Jul 11, 2022，Views：636

Original protocol used in research article：

Single-nucleus transcriptomic analysis of human dorsal root ganglion neurons
eLife, Nov 26, 2021; DOI: 10.7554/eLife.71752

Validation and Survival Analysis of Hub Genes

Authors: Yanan Wang and Jiguang Guo
Date: Jul 10, 2022，Views：534

Original protocol used in research article：

Identification of Down-Regulated ADH1C is Associated With Poor Prognosis in Colorectal Cancer Using Bioinformatics Analysis
Frontiers in Molecular Biosciences, Mar 1, 2022; DOI: 10.3389/fmolb.2022.791249

DNA Extraction and Sequencing

Authors: Changquan Zhang and Yadong Guo
Date: Jul 10, 2022，Views：572

Original protocol used in research article：

Characterization and Comparative Analysis of Mitochondrial Genomes Among the Calliphoridae (Insecta: Diptera: Oestroidea) and Phylogenetic Implications
Frontiers in Genetics, Feb 17, 2022; DOI: 10.3389/fgene.2022.799203

Coulter counter measurement

Authors: Douaa Mugahid and Marc W Kirschner
Date: Jul 10, 2022，Views：566

Original protocol used in research article：

YAP regulates cell size and growth dynamics via non-cell autonomous mediators
eLife, Jan 9, 2020; DOI: 10.7554/eLife.53404

Immunofluorescence microscopy and analysis

Authors: Michèle MG Hillege, Andi Shi and Richard T Jaspers
Date: Jul 10, 2022，Views：550

Original protocol used in research article：

Lack of Tgfbr1 and Acvr1b synergistically stimulates myofibre hypertrophy and accelerates muscle regeneration
eLife, ; DOI: 10.7554/eLife.77610

Ex vivo OT-1 cross-priming assay and secreted cytokine measurement

Authors: Rodney Cheng-En Hsieh and Michael A. Curran
Date: Jul 9, 2022，Views：592

Original protocol used in research article：

ATR-mediated CD47 and PD-L1 up-regulation restricts radiotherapy-induced immune priming and abscopal responses in colorectal cancer
Science Immunology, Jun 10, 2022; DOI: 10.1126/sciimmunol.abl9330

Bulk RNA-seq

Authors: Thanh Hoang, Jie Wang, David R. Hyde, Jiang Qian, Seth Blackshaw and Patrick Boyd
Date: Jul 8, 2022，Views：602

Original protocol used in research article：

Gene regulatory networks controlling vertebrate retinal regeneration
Science, Oct 1, 2020; DOI: 10.1126/science.abb8598

Histological examination

Authors: Francesco Zorzato and Alexis Ruiz
Date: Jul 8, 2022，Views：587

Original protocol used in research article：

Improvement of muscle strength in a mouse model for congenital myopathy treated with HDAC and DNA methyltransferase inhibitors
eLife, Mar 3, 2022; DOI: 10.7554/eLife.73718

Adoptive transfer

Authors: Federica Piattini and Manfred Kopf
Date: Jul 8, 2022，Views：567

Original protocol used in research article：

Monocyte-derived alveolar macrophages autonomously determine severe outcome of respiratory viral infection
Science Immunology, Jul 1, 2022; DOI: 10.1126/sciimmunol.abj5761

Study design

Authors: Tian-tuo Zhang, Hui Li, Bo Peng and Xuan-xian Peng
Date: Jul 7, 2022，Views：581

Original protocol used in research article：

Glutamine promotes antibiotic uptake to kill multidrug-resistant uropathogenic bacteria
Science Translational Medicine, Dec 22, 2021; DOI: 10.1126/scitranslmed.abj0716

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