Protocols shared by authors via RaP service
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Isolation and characterization of mEVs and EV-depleted supernatant
Authors: Lingjun Tong, Jiong-Wei Wang and Sitong Zhang
Date: Oct 26, 2023，Views：1101
Original protocol used in research article：
Milk-derived extracellular vesicles protect intestinal barrier integrity in the gut-liver axis
Science Advances, Apr 12, 2023; DOI: 10.1126/sciadv.ade5041
Protein extraction and Macf1 identification by western blot
Authors: Alireza Ghasemizadeh and Vincent Gache
Date: Oct 26, 2023，Views：1117
Original protocol used in research article：
MACF1 controls skeletal muscle function through the microtubule-dependent localization of extra-synaptic myonuclei and mitochondria biogenesis
eLife, Aug 27, 2021; DOI: 10.7554/eLife.70490
Conjugate formation and processing for ultrastructural studies
Authors: Jane C. Stinchcombe and Gillian M. Griffiths
Date: Oct 25, 2023，Views：739
Original protocol used in research article：
Ectocytosis renders T cell receptor signaling self-limiting at the immune synapse
Science, May 25, 2023; DOI: 10.1126/science.abp8933
KIF22 Immunofluorescence
Author: Jason Stumpff
Date: Oct 22, 2023，Views：778
Original protocol used in research article：
Pathogenic mutations in the chromokinesin KIF22 disrupt anaphase chromosome segregation
eLife, ; DOI: 10.7554/eLife.78653
Lentiviral transductions and engineering of stable cell lines
Authors: Kok-Siong Chen and Khalid Shah
Date: Oct 19, 2023，Views：704
Original protocol used in research article：
Bifunctional cancer cell–based vaccine concomitantly drives direct tumor killing and antitumor immunity
Science Translational Medicine, Jan 4, 2023; DOI: 10.1126/scitranslmed.abo4778
Electroporation of CD34+ cells
Authors: Kumarasamypet M Mohankumar and Nithin Sam R
Date: Oct 19, 2023，Views：684
Original protocol used in research article：
Identification of novel HPFH-like mutations by CRISPR base editing that elevate the expression of fetal hemoglobin
eLife, Feb 11, 2022; DOI: 10.7554/eLife.65421
Electrode holder implantation and transcranial direct current stimulation (tDCS)
Authors: Zheng Gan and Rohini Kuner
Date: Oct 15, 2023，Views：57
Original protocol used in research article：
Layer-specific pain relief pathways originating from primary motor cortex
Science, Dec 22, 2022; DOI: 10.1126/science.add4391
Animal models
Authors: Sitong Zhang, Lingjun Tong, Huaxi Yi and Jiong-Wei Wang
Date: Oct 12, 2023，Views：521
Original protocol used in research article：
Milk-derived extracellular vesicles protect intestinal barrier integrity in the gut-liver axis
Science Advances, Apr 12, 2023; DOI: 10.1126/sciadv.ade5041
gClustering and phylogenetic analyses
Authors: Mariana G López and Iñaki Comas
Date: Oct 9, 2023，Views：347
Original protocol used in research article：
Population-based sequencing of Mycobacterium tuberculosis reveals how current population dynamics are shaped by past epidemics
eLife, Jul 26, 2022; DOI: 10.7554/eLife.76605
Detection and Analysis of human ALDH2 by quantitative RT-PCR
Authors: Yao-Hui Gao, Guo-Qiang Chen and Li-Shun Wang
Date: Oct 7, 2023，Views：346
Original protocol used in research article：
VHL deficiency augments anthracycline sensitivity of clear cell renal cell carcinomas by down-regulating ALDH2
Nature Communications, Jun 15, 2017; DOI: 10.1038/ncomms15337
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