Bio-101 - Improve Research Reproducibility

Determination of total triterpenoid content

Authors: Marija R. Mandić, Bojan Dj. Božić and Biljana Dj. Božić Nedeljković
Date: Nov 9, 2023，Views：58

Original protocol used in research article：

Chemical characterization and in vitro immunomodulatory effects of different extracts of moss Hedwigia ciliata (Hedw.) P. Beauv. from the Vršačke Planine Mts., Serbia
PLoS ONE, Feb 11, 2021; DOI: 10.1371/journal.pone.0246810

Pseudovirus (PSV) Assay

Authors: Thomas F. Rogers, Devin Sok, Joseph G. Jardine and Dennis R. Burton
Date: Nov 6, 2023，Views：2341

Original protocol used in research article：

Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model
Science, Aug 21, 2020; DOI: 10.1126/science.abc7520

Determination of site‐specific m6A modification levels using SELECT

Author: Tian‐Liang Xia
Date: Nov 4, 2023，Views：1835

Original protocol used in research article：

N(6)‐methyladenosine‐binding protein YTHDF1 suppresses EBV replication and promotes EBV RNA decay
EMBO Reports, Feb 19, 2021; DOI: 10.15252/embr.202050128

Pharyngeal Pumping Assay (Drosophila melanogaster)

Authors: John R Carlson and Ryan M Joseph
Date: Nov 3, 2023，Views：1619

Original protocol used in research article：

A receptor and neuron that activate a circuit limiting sucrose consumption
eLife, Mar 23, 2017; DOI: 10.7554/eLife.24992

2.4. Amoeba–Bacteria Co-Culture

Authors: Mohamed Shaheen and Nicholas J. Ashbolt
Date: Nov 2, 2023，Views：1549

Original protocol used in research article：

Differential Bacterial Predation by Free-Living Amoebae May Result in Blooms of Legionella in Drinking Water Systems
Microorganisms, Jan 15, 2021; DOI: 10.3390/microorganisms9010174

Isolation and characterization of mEVs and EV-depleted supernatant

Authors: Lingjun Tong, Jiong-Wei Wang and Sitong Zhang
Date: Oct 26, 2023，Views：1100

Original protocol used in research article：

Milk-derived extracellular vesicles protect intestinal barrier integrity in the gut-liver axis
Science Advances, Apr 12, 2023; DOI: 10.1126/sciadv.ade5041

Protein extraction and Macf1 identification by western blot

Authors: Alireza Ghasemizadeh and Vincent Gache
Date: Oct 26, 2023，Views：1107

Original protocol used in research article：

MACF1 controls skeletal muscle function through the microtubule-dependent localization of extra-synaptic myonuclei and mitochondria biogenesis
eLife, Aug 27, 2021; DOI: 10.7554/eLife.70490

Conjugate formation and processing for ultrastructural studies

Authors: Jane C. Stinchcombe and Gillian M. Griffiths
Date: Oct 25, 2023，Views：739

Original protocol used in research article：

Ectocytosis renders T cell receptor signaling self-limiting at the immune synapse
Science, May 25, 2023; DOI: 10.1126/science.abp8933

KIF22 Immunofluorescence

Author: Jason Stumpff
Date: Oct 22, 2023，Views：777

Original protocol used in research article：

Pathogenic mutations in the chromokinesin KIF22 disrupt anaphase chromosome segregation
eLife, ; DOI: 10.7554/eLife.78653

Lentiviral transductions and engineering of stable cell lines

Authors: Kok-Siong Chen and Khalid Shah
Date: Oct 19, 2023，Views：703

Original protocol used in research article：

Bifunctional cancer cell–based vaccine concomitantly drives direct tumor killing and antitumor immunity
Science Translational Medicine, Jan 4, 2023; DOI: 10.1126/scitranslmed.abo4778

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