Bio-101 - Improve Research Reproducibility

Immunofluorescence, immunoprecipitation, and ELISA using the Scicons anti-dsRNA J2 antibody

Authors: Elizabeth Ochoa and Bess Frost
Date: Jun 22, 2023，Views：961

Original protocol used in research article：

Pathogenic tau–induced transposable element–derived dsRNA drives neuroinflammation
Science Advances, Jan 6, 2023; DOI: 10.1126/sciadv.abq5423

APEX2 biotinylation in brain slices

Authors: David Sulzer and Peter A Sims
Date: Jun 21, 2023，Views：942

Original protocol used in research article：

Subcellular proteomics of dopamine neurons in the mouse brain
eLife, Jan 31, 2022; DOI: 10.7554/eLife.70921

CUT&RUN library preparation

Author: Paolo Emanuele Forni
Date: Jun 20, 2023，Views：53

Original protocol used in research article：

Sociosexual behavior requires both activating and repressive roles of Tfap2e/AP-2ε in vomeronasal sensory neurons
eLife, ; DOI: 10.7554/eLife.77259

Sortase A conjugation of HIV-1 envelope trimers to ferritin nanoparticles

Author: Kevin O. Saunders
Date: Jun 13, 2023，Views：920

Original protocol used in research article：

Targeted selection of HIV-specific antibody mutations by engineering B cell maturation
Science, Dec 5, 2019; DOI: 10.1126/science.aay7199

crystal fish imaging

Authors: Francois Kroll and Jason Rihel
Date: Jun 13, 2023，Views：880

Original protocol used in research article：

A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes
eLife, Jan 8, 2021; DOI: 10.7554/eLife.59683

2.3.5. The Visual Analog Scale for Irritable Bowel Syndrome Questionnaire

Authors: Clara Nilholm and Bodil Ohlsson
Date: Jun 11, 2023，Views：14

Original protocol used in research article：

Assessment of a 4-Week Starch- and Sucrose-Reduced Diet and Its Effects on Gastrointestinal Symptoms and Inflammatory Parameters among Patients with Irritable Bowel Syndrome
Nutrients, Jan 28, 2021; DOI: 10.3390/nu13020416

CUMS model

Authors: Shu Yan Yu, Ye Li, Cuiqin Fan and Changmin Wang
Date: Jun 11, 2023，Views：979

Original protocol used in research article：

Stress-induced reduction of Na+/H+ exchanger isoform 1 promotes maladaptation of neuroplasticity and exacerbates depressive behaviors
Science Advances, Nov 11, 2022; DOI: 10.1126/sciadv.add7063

Generation of SC2-VLPs - Protocol for transfection and filtering in 96well format

Authors: Melanie Ott and Jennifer A. Doudna
Date: Jun 11, 2023，Views：1037

Original protocol used in research article：

Rapid assessment of SARS-CoV-2 evolved variants using virus-like particles
Science, Nov 4, 2021; DOI: 10.1126/science.abl6184

Luciferase-based cytotoxicity assay

Authors: Gernot Stuhler and Boris Nowotny
Date: Jun 8, 2023，Views：1507

Original protocol used in research article：

Combinatorial targeting of multiple myeloma by complementing T cell engaging antibody fragments
Communications Biology, Jan 8, 2021; DOI: 10.1038/s42003-020-01558-0

Tissue processing and staining

Authors: Arnau Sans-Dublanc and Carlos Sindreu
Date: Jun 6, 2023，Views：781

Original protocol used in research article：

Septal GABAergic inputs to CA1 govern contextual memory retrieval
Science Advances, Oct 29, 2020; DOI: 10.1126/sciadv.aba5003

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