Protocols shared by authors via RaP service
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Interpretation of Fractional Inhibitory Concentration Index (FICI)
Authors: Wenli Feng and Jing Yang
Date: Sep 1, 2023，Views：707
Original protocol used in research article：
Cotreatment with Aspirin and Azole Drugs Increases Sensitivity of Candida albicans in vitro
Infect Drug Resist, Jun 2, 2021; DOI: 10.2147/IDR.S314538
Quantitative polymerase chain reaction (qPCR)
Authors: Ke-Hsuan Wei, Shih-Lei (Ben) Lai and I-Ting Lin
Date: Aug 31, 2023，Views：177
Original protocol used in research article：
Comparative single-cell profiling reveals distinct cardiac resident macrophages essential for zebrafish heart regeneration
eLife, Jul 27, 2023; DOI: 10.7554/eLife.84679
Cytokine release assay
Authors: Vanaja Konduri and William K. Decker
Date: Aug 31, 2023，Views：197
Original protocol used in research article：
A subset of cytotoxic effector memory T cells enhances CAR T cell efficacy in a model of pancreatic ductal adenocarcinoma
Science Translational Medicine, May 5, 2021; DOI: 10.1126/scitranslmed.abc3196
2.8. Mitochondrial membrane potential (ΔΨm) and mtROS
Authors: Sehwan Jang and Sabzali Javadov
Date: Aug 31, 2023，Views：202
Original protocol used in research article：
Elucidating the contribution of mitochondrial glutathione to ferroptosis in cardiomyocytes
Redox Biology, Jun 1, 2021; DOI: 10.1016/j.redox.2021.102021
Tryptophan-quenching experiments
Author: Krishnananda Chattopadhyay
Date: Aug 29, 2023，Views：230
Original protocol used in research article：
The metal cofactor zinc and interacting membranes modulate SOD1 conformation-aggregation landscape in an in vitro ALS model
eLife, Apr 7, 2021; DOI: 10.7554/eLife.61453
En-bloc sample preparation for 3D electron microscopy
Authors: Ali Karimi and Moritz Helmstaedter
Date: Aug 29, 2023，Views：49
Original protocol used in research article：
Cell-type specific innervation of cortical pyramidal cells at their apical dendrites
eLife, Feb 28, 2020; DOI: 10.7554/eLife.46876
Efferocytosis assay
Authors: Zihao Yao and Haiyan Zhang
Date: Aug 27, 2023，Views：441
Original protocol used in research article：
Down-regulated GAS6 impairs synovial macrophage efferocytosis and promotes obesity-associated osteoarthritis
eLife, May 5, 2023; DOI: 10.7554/eLife.83069
MNase assay with EZ Nucleosomal DNA prep Kit
Authors: Yu-Xuan Lu and Linda Partridge
Date: Aug 20, 2023，Views：392
Original protocol used in research article：
A TORC1-histone axis regulates chromatin organisation and non-canonical induction of autophagy to ameliorate ageing
eLife, May 14, 2021; DOI: 10.7554/eLife.62233
Generation of pembrolizumab scFv
Authors: Zachary Davis and Jeffrey S. Miller
Date: Aug 16, 2023，Views：30
Original protocol used in research article：
Low-density PD-1 expression on resting human natural killer cells is functional and upregulated after transplantation
Blood Advances, Feb 18, 2021; DOI: 10.1182/bloodadvances.2019001110
HaloTag labeling
Author: Benjamin S Glick
Date: Aug 11, 2023，Views：519
Original protocol used in research article：
A microscopy-based kinetic analysis of yeast vacuolar protein sorting
eLife, Jun 25, 2020; DOI: 10.7554/eLife.56844
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