Protocols shared by authors via RaP service
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Proteomics and untargeted metabolomics from plasma
Author: Kazuo Ando
Date: Jul 17, 2023，Views：931
Original protocol used in research article：
Integrated trajectories of the maternal metabolome, proteome, and immunome predict labor onset
Science Translational Medicine, May 5, 2021; DOI: 10.1126/scitranslmed.abd9898
Quantitative real-time PCR analysis
Author:
Date: Jul 15, 2023，Views：127
Original protocol used in research article：
ULK1-mediated metabolic reprogramming regulates Vps34 lipid kinase activity by its lactylation
Science Advances, Jun 2, 2023; DOI: 10.1126/sciadv.adg4993
2.8. Observations of seed germination and seedling growth parameters
Author: Saiqa Andleeb
Date: Jul 15, 2023，Views：39
Original protocol used in research article：
Efficiency of cow dung based vermi-compost on seed germination and plant growth parameters of Tagetes erectus (Marigold)
Heliyon, Jan 6, 2021; DOI: 10.1016/j.heliyon.2020.e05895
Nystatin and subunit vaccine injection in mice
Author: Beth Ann Jiron Tamburini
Date: Jul 14, 2023，Views：971
Original protocol used in research article：
Molecular tracking devices quantify antigen distribution and archiving in the murine lymph node
eLife, Apr 12, 2021; DOI: 10.7554/eLife.62781
High-resolution 3D tissue imaging
Authors: Hua Zhang, Lu Mu, Xueqiang Xu, Xuebing Yang and Yan Zhang
Date: Jul 14, 2023，Views：1000
Original protocol used in research article：
Imaging and tracing the pattern of adult ovarian angiogenesis implies a strategy against female reproductive aging
Science Advances, Jan 12, 2022; DOI: 10.1126/sciadv.abi8683
Generation of SH-SY5Y HTT knockout cell lines by CRISPR-Cas9
Author: Jianning Wei
Date: Jul 13, 2023，Views：967
Original protocol used in research article：
RNA-seq analysis reveals significant transcriptome changes in huntingtin-null human neuroblastoma cells
BMC Medical Genomics, Jul 2, 2021; DOI: 10.1186/s12920-021-01022-w
HepaRG culture
Author: Francesco Bellanti
Date: Jul 13, 2023，Views：873
Original protocol used in research article：
Inhibition of nuclear factor (erythroid-derived 2)-like 2 promotes hepatic progenitor cell activation and differentiation
NPJ Regenerative Medicine, May 26, 2021; DOI: 10.1038/s41536-021-00137-z
MAG Bead DNA Extraction Protocol
Authors: Tamara D. Simon and Christopher E. Pope
Date: Jul 11, 2023，Views：730
Original protocol used in research article：
Characterization of cerebrospinal fluid (CSF) microbiota from patients with CSF shunt infection and reinfection using high throughput sequencing of 16S ribosomal RNAgenes
PLoS ONE, Jan 6, 2021; DOI: 10.1371/journal.pone.0244643
CyTOF analysis
Author: Chu-Xia Deng
Date: Jul 11, 2023，Views：725
Original protocol used in research article：
Cullin-5 deficiency orchestrates the tumor microenvironment to promote mammary tumor development through CREB1-CCL2 signaling
Science Advances, Jan 20, 2023; DOI: 10.1126/sciadv.abq1395
Analysis of differential expression in tumor and matched normal tissues
Author: Ying Yuan
Date: Jul 1, 2023，Views：746
Original protocol used in research article：
Pan-cancer analyses demonstrate that ANKRD6 is associated with a poor prognosis and correlates with M2 macrophage infiltration in colon cancer
Chinese Journal of Cancer Research, Feb 28, 2021; DOI: 10.21147/j.issn.1000-9604.2021.01.10
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