Protocols shared by authors via RaP service
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EGFR Internalization and EGFR Recycling Assays
Authors: Chia-Hsing Shen and Li-Chung Hsu
Date: May 17, 2023，Views：679
Original protocol used in research article：
ZNRF1 Mediates Epidermal Growth Factor Receptor Ubiquitination to Control Receptor Lysosomal Trafficking and Degradation
Frontiers in Cell and Developmental Biology, Apr 29, 2021; DOI: 10.3389/fcell.2021.642625
Denaturing in vivo ubiquitination assay
Authors: Tyler B Faust, Amit Weiss, Bhargavi Jayaraman, Billy W Newton, Nevan J Krogan, Iván D'Orso and Alan D Frankel
Date: May 16, 2023，Views：710
Original protocol used in research article：
The HIV-1 Tat protein recruits a ubiquitin ligase to reorganize the 7SK snRNP for transcriptional activation
eLife, May 30, 2018; DOI: 10.7554/eLife.31879
RNAseq
Authors: Laura Amado-Rodríguez and Guillermo M Albaiceta
Date: May 16, 2023，Views：33
Original protocol used in research article：
Effects of IFIH1 rs1990760 variants on systemic inflammation and outcome in critically ill COVID-19 patients in an observational translational study
eLife, Jan 21, 2022; DOI: 10.7554/eLife.73012
The Transgression-Related Interpersonal Motivations Inventory (TRIM-18; McCullough et al., 1998, 2006)
Author: Pilar Martinez-Diaz
Date: May 16, 2023，Views：16
Original protocol used in research article：
Victim's Perspective of Forgiveness Seeking Behaviors After Transgressions
Frontiers in Psychology, Apr 21, 2021; DOI: 10.3389/fpsyg.2021.656689
Intracellular cytokine staining
Authors: Kizzmekia S. Corbett, Barney S. Graham and Robert A. Seder
Date: May 15, 2023，Views：475
Original protocol used in research article：
Immune correlates of protection by mRNA-1273 vaccine against SARS-CoV-2 in nonhuman primates
Science, Jul 29, 2021; DOI: 10.1126/science.abj0299
Protein expression and purification
Authors: Donghyuk Shin and Ivan Dikic
Date: May 14, 2023，Views：566
Original protocol used in research article：
Bacterial OTU deubiquitinases regulate substrate ubiquitination upon Legionella infection
eLife, Nov 13, 2020; DOI: 10.7554/eLife.58277
Mendelian randomization and sensitivity analyses
Author: Guillaume Paré
Date: May 14, 2023，Views：64
Original protocol used in research article：
GWAS and ExWAS of blood mitochondrial DNA copy number identifies 71 loci and highlights a potential causal role in dementia
eLife, Jan 13, 2022; DOI: 10.7554/eLife.70382
Protein expression in PAO1 and cells lysis
Authors: Lital Alfonta and Eden Ozer
Date: May 14, 2023，Views：346
Original protocol used in research article：
An inside look at a biofilm: Pseudomonas aeruginosa flagella biotracking
Science Advances, Jun 11, 2021; DOI: 10.1126/sciadv.abg8581
BUT measurement and corneal damage scoring
Authors: Masatoshi Miyamoto, Takayuki Sassa and Akio Kihara
Date: May 13, 2023，Views：411
Original protocol used in research article：
Lipid polarity gradient formed by ω-hydroxy lipids in tear film prevents dry eye disease
eLife, Apr 7, 2020; DOI: 10.7554/eLife.53582
DR-GFP reporter assay
Authors: Calvin Shun Yu Lo and Nitika Taneja
Date: May 12, 2023，Views：550
Original protocol used in research article：
SMARCAD1-mediated active replication fork stability maintains genome integrity
Science Advances, May 5, 2021; DOI: 10.1126/sciadv.abe7804
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