Protocols shared by authors via RaP service
Journal
3.5.2. TBARS Assay
Authors: Fatma M. Elessawy and Randy W. Purves
Date: May 24, 2023，Views：959
Original protocol used in research article：
An Untargeted Metabolomics Approach for Correlating Pulse Crop Seed Coat Polyphenol Profiles with Antioxidant Capacity and Iron Chelation Ability
Molecules, Jun 23, 2021; DOI: 10.3390/molecules26133833
EdU labeling assay
Authors: Akansha M. Shah, Eric N. Olson and Ning Liu
Date: May 23, 2023，Views：847
Original protocol used in research article：
TWIST2-mediated chromatin remodeling promotes fusion-negative rhabdomyosarcoma
Science Advances, Apr 28, 2023; DOI: 10.1126/sciadv.ade8184
Protein purification, MST, and EMSAs
Authors: Shengze Yao and Yi Li
Date: May 23, 2023，Views：734
Original protocol used in research article：
The key micronutrient copper orchestrates broad-spectrum virus resistance in rice
Science Advances, Jul 1, 2022; DOI: 10.1126/sciadv.abm0660
Purification of DNA Origami Nanostructures
Authors: Pierre Stömmer and Hendrik Dietz
Date: May 23, 2023，Views：723
Original protocol used in research article：
Cryo-Electron Microscopy and Mass Analysis of Oligolysine-Coated DNA Nanostructures
ACS Nano, Mar 16, 2021; DOI: 10.1021/acsnano.0c10137
Data acquisition of resting-state fMRI and optogenetic fMRI experiments
Authors: Jiaming Liu, Ed X. Wu, Yanqiu Feng and Xiong Cao
Date: May 23, 2023，Views：625
Original protocol used in research article：
Astrocyte dysfunction drives abnormal resting-state functional connectivity in depression
Science Advances, Nov 16, 2022; DOI: 10.1126/sciadv.abo2098
Macrophage depletion and reconstitution
Authors: Kang Yi Su and Wei-Jia Luo
Date: May 23, 2023，Views：578
Original protocol used in research article：
HLJ1 amplifies endotoxin-induced sepsis severity by promoting IL-12 heterodimerization in macrophages
eLife, ; DOI: 10.7554/eLife.76094
PLZF knockdown using siRNA
Author: Shuchi Agrawal Singh
Date: May 22, 2023，Views：543
Original protocol used in research article：
PLZF targets developmental enhancers for activation during osteogenic differentiation of human mesenchymal stem cells
eLife, Jan 23, 2019; DOI: 10.7554/eLife.40364
Generation of nanobody dimers by sortase-mediated functionalization and click chemistry
Author: Leo Hanke
Date: May 22, 2023，Views：549
Original protocol used in research article：
Multivariate mining of an alpaca immune repertoire identifies potent cross-neutralizing SARS-CoV-2 nanobodies
Science Advances, Mar 25, 2022; DOI: 10.1126/sciadv.abm0220
Differentiation of rTTA/Ngn2-positive PSCs to iNeurons
Authors: Maria Schörnig, Valentina Rava and Elena Taverna
Date: May 22, 2023，Views：616
Original protocol used in research article：
Comparison of induced neurons reveals slower structural and functional maturation in humans than in apes
eLife, Jan 20, 2021; DOI: 10.7554/eLife.59323
Sequences (Figs 1-3)
Authors: Morgane Rolland and Eric Lewitus
Date: May 17, 2023，Views：22
Original protocol used in research article：
Design of a pan-betacoronavirus vaccine candidate through a phylogenetically informed approach
Science Advances, Jan 18, 2023; DOI: 10.1126/sciadv.abq4149
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